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Öğe Clinoptilolite supported feeding reduces excessive iron in thalassemia rat model created with iron loading(2021) Dar, Kadriye Akgün; Kapucu, Ayşegül; Kuruca, Serap; Özerkan, DilşadThere is no regulatory mechanism for the removal of iron that accumulates in the body. Thalassemia patients are most affected by iron overload. The method often used in the treatment of these patients is iron chelation therapy, which involves removing excess iron from the bloodstream, but it is insufficient. Nutritional supplements and herbal remedies are complementary tools that may help improve the health of an individual with iron load. Here, we tried to develop a method that affecting intestinal absorption in an animal model with clinoptilolite feeding to reduce the iron load in the circulation. 32 rats were divided into 4 groups as control, Cli, Iron, Cli+Iron. Iron and Cli+Iron groups received iron at a dose of 250 mg/kg/day for 10 days, and Cli and Cli+Iron groups were fed a diet with 50% clinoptilolite for one month. Histological preparation and Fe2+, Cu2+, Zn2+ measurements were done in all tissues. Consequently, clinoptilolite significantly lowered the iron level in the stomach. On the contrary, iron absorption was increased in the small intestine, but iron transportation to the blood was decreased by clinoptilolite. The iron levels of clinoptilolite groups (Cli and Cli+Iron) were reduced in the tissues of heart, lung, liver, kidney, and spleen because of the different level of iron necessities of each tissue compared to the group with iron overload. The clinoptilolite could preserve organs against iron toxicity by enhancing the absorption of iron in the small intestine but lowering the iron level in blood. Even though there was no detailed information regarding the mechanism of reduction iron overload by the clinoptilolite, serum and chyme could help to make some helpful inferences to elucidate the mechanism of iron chelation.Öğe Distribution of spleen connective tissue fibers in diabetic and vitamin C treated diabetic rats(Taylor & Francis Ltd, 2020) Özerkan, Dilşad; Özsoy, Nesrin; Cebesoy, Suna; Özer, ÇiğdemWe investigated the distribution of connective tissue fibers in diabetic and vitamin C treated diabetic rat spleen. Rats were divided into three groups: group A, control; group B, diabetic; group C, vitamin C treated diabetic. Diabetes was induced by streptozotocin. Vitamin C was administered intragastrically for 21 days. Spleen tissues were examined by light microscopy after staining with Masson's trichrome, Gomori silver impregnation and van Gieson. In group B, we found accumulation of collagen fibers in the trabeculae, in the capsule and around the central artery and splenic sinusoids. Splenic cord thickening due to fibrosis was observed. Reticular fibers accumulated principally in the white and red pulps of the spleen and focal reticular fiber thickening was observed in the dense fiber areas. Partial elastic fiber rupture was observed among the fibers of the elastic lamina of the arteries in the hilum. By contrast, the distribution of collagen fibers in group C was similar to group A. Collagen fiber accumulation was decreased in group C compared to group B. We found little reticular fiber thickening in group C and elastic fibers maintained their integrity and were better organized than in group B. Our findings suggest thatappropriate doses of vitamin C may exert beneficial effects on the structure of the connective tissue fibers in the diabetic spleen.Öğe Some bryophytes trigger cytotoxicity of stem cell-like population in 5-fluorouracil resistant colon cancer cells(Taylor and Francis, 2021) Özerkan, Dilşad; Erol, Ayşe; Altuner, Ergin Murat; Canlı, Kerem; Kuruca, Dürdane SerapColorectal cancer is the third most common cancer worldwide. Cancer stem cells are known to play an important role in relapse, and metastases of the disease after chemotherapy. Investigation of new drugs, and their combinations targeting these cells and thus eliminating cancer is one of the most urgent needs of today’s chemotherapy. The aim of the present study was to evaluate the effects of Bryophytes like Abietinella abietina (AA), Homolothecium sericeum (HS), Tortella tortuosa (TT), Syntrichia ruralis (SR), and Bryoerythrophyllum rubrum (BR) species extracted with ethyl alcohol on 5-fluorouracil(5-FU) resistant colorectal cancer cell lines (HCT116 and HT29). After extraction, stock solutions of bryophytes were prepared, and IC50 values were detected in drug-resistant cells obtained with 5-FU application. CD24+, CD44+/CD133+ surface markers and P?glycoprotein (P-gp) mediated efflux were isolated from both 5-FU treated cells and analyzed using the flow cytometry. In all bryophyte-treated groups, the binding Rho123low (low Rho fluorescence) and Rhohigh (high Rho fluorescence) were sorted from 5-FU resistant HCT116, and HT-29 cells. All types of bryophytes were found cytotoxic. Bryophyte extract reduced the percentage of Rholow cells in cultures incubated with 5-FU. In summary, the implementation of these bryophytes might be regarded as an effective approach for treatment of colorectal cancer due to their cytotoxic effect that decreases the recurrence of the disease.Öğe Syntrichia ruraliformis (Besch.) Mans., Hypnum andoi A.J.E.Sm. ve Platyhypnidium riparioides Dixon Etanol Ekstraktlarının HCT116 Hücre Canlılığı Üzerindeki Etkilerinin Spektroskopik Açıdan İncelenmesi(2021) Canlı, Kerem; Altuner, Ergin Murat; Özerkan, Dilşad; Zengin Köksal, HilalKolon kanseri neredeyse her yıl bir milyondan fazla yeni tanıların ortaya koyulduğu ve dünyada en sık rastlanan üçüncü kanser türüdür. Her yaş grubunda görülebildiği gibi kalıtımsal kökenli değildir. Bu veriler göz önüne alındığında kanserin tedavisi için yeni ilaç kombinasyonlarının araştırılması önemli bir ihtiyaçtır. Bu çalışmada, anti-kanser aktivitesi bilinmeyen Syntrichia ruraliformis (Besch.) Mans., Hypnum andoi A.J.E.Sm, Platyhypnidium riparioides Dixon karayosunu türlerinden elde edilen ekstraktlarının HCT116 kolon kanser hücre hatlarına karşı etkileri değerlendirilmiştir. Karayosunu türlerinin etil alkol çözücüsünde elde edilen ekstrelerinden, DMSO ile stok solüsyonlar hazırlanarak, çeşitli konsantrasyonlarda sitotoksite test (MTT) deneyleri yapılmıştır. HCT116 serisinde bulunan tüm karayosunu türlerinin IC50 değerlerine göre belirlenen dozlar aynı şekilde mezenkimal kök hücrelere (MKH) uygulanmıştır. Ayrıca, Fourier transform kızılötesi (FTIR) spektroskopisi üç karayosununa maruz kalan kanser hücrelerini analiz etmek için kullanılmıştır. Sonuç olarak, karayosunu türlerinin HCT116 kolorektal kanser hücre hattına karşı yüksek oranda antiproliferatif etki gösterdiği, bunun aksine MKH hücrelerinde letal olmadığı tespit edilmiştir. Ayrıca, FTIR spektrumu ile elde edilen sonuçların tüm hücre proliferasyonu verileriyle paralel seyrettiği moleküler düzeyde de belirlenmiştir. Tüm sonuçların ışığında bu karayosunu türlerinin, biyoaktif bileşenleri belirlendiği takdirde, potansiyel kanser ilacı olma özelliğine sahip olduğu düşünülmektedir. Anahtar kelimeler: Briyofit, anti-proliferatif, kolorektal kanser, FTIR
