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Öğe The effect of lidocaine on TRPM 2,6,7 and 8 channels in liver ischemia / reperfusion model in rats(BAYRAKOL MEDICAL PUBLISHER, 2020) Bilecik, Tuna; Özozan, Ömer Vefik; Gökçe, HasanAim: The transient receptor potential melastatin (TRPM) channel is subfamilies of the transient receptor potential (TRP) channels, cation permeable channels. Ischemia and reperfusion (I / R) injury is a phenomenon highlighting cellular damage of the liver caused by oxygen. Lidocaine is a local anesthetic that blocks sodium channels and suppresses mitochondrial functions of neutrophils. In this study, we purpose to investigate the effects of TRPM2/8 and TRPM6/7 expressions after lidocaine treatment in liver ischemia/reperfusion rat model. Materials and Methods: The study was carried out on 32 male Wistar rats. The animals were randomly divided into 4 groups including sham, lidocaine, I/R group and I/R- lidocaine group. The portal vein and the hepatic artery branches were clamped for 60 minutes for complete ischemia. TRPM2/8 and TRPM6/7 gene expression levels were assessed by RT-qPCR. Results: The expression levels of TRPM2 were significantly higher in liver-I/R group compared to sham and lidocaine groups (p<0.05, p<0.000, respectively), TRPM8 genes were significantly higher in liver-I/R group compared to sham and lidocaine groups (p<0.05, p<0.05, respectively). However, the expression levels of TRPM6 gene was significantly higher in liver-I/R group compared to sham, lidocaine, and I/R-lidocaine groups (p<0.05, p<0.01, p<0.05, respectively), TRPM7 genes was significantly higher in liver-I/R group compared to sham, lidocaine and I/R-lidocaine groups (p<0.05, p<0.000, p<0.05, respectively). Discussion: In conclusion, we firstly showed that an association between the expression level of TRPM2/8, TRPM6/7, and hepatic I/R, I/R-lidocaine groups as well as TRPM2/8 and TRPM6/7 gene expressions are affected by lidocaine in the liver-I/R in a rat model.Öğe The effects of TRPM2, TRPM6, TRPM7 and TRPM8 gene expression in hepatic ischemia reperfusion injury(Verduci Publisher, 2019) Bilecik, Tuna; Karateke, F.; Elkan, H.; Gokce, H.OBJECTIVE: Mammalian transient receptor potential melastatin (TRPM) channels are a form of calcium channels and they transport calcium and magnesium ions. TRPM has eight subclasses including TRPM1-8. TRPM2, TRPM6, TRPM7, TRPM8 are expressed especially in the liver cell. Therefore, we aim to investigate the effects of TRPM2, TRPM6, TRPM7, and TRPM8 gene expression and histopathologic changes after treatment of verapamil in the hepatic ischemia-reperfusion rat model. MATERIALS AND METHODS: Animals were randomly assigned to one or the other of the following groups including sham (n=8) group, verapamil (calcium entry blocker) (n=8) group, I/R group (n=8) and I/R-verapamil (n=8) group. TRPM 2, 6, 7, 8 gene expression level was assessed by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) and histopathologic changes were determined by the hematoxylin and eosin (HE) examination. RESULTS: The expression level of TRPM 2, 6, 7, and 8 genes was significantly higher in ischemia- reperfusion (I/R), verapamil, IR-verapamil groups compared to sham group. The p-values were 0.0024, < 0.0001, 0.0002, 0.006 for TRPM2, TRPM6, TRPM7, and TRPM8, respectively. Severe necrotic, degenerative differentiations and severe hemorrhagic areas were observed in hepatocytes from IR group. Also, moderate necrotic and degenerative differentiations and moderate hemorrhagic areas were observed in hepatocytes from IR-verapamil group. CONCLUSIONS: This is the first study reporting an association between the expression level of TRPM 2, 6, 7, 8 in a hepatic ischemia-reperfusion rat model. Moreover, TRPM 2, 6, 7, 8 affect hepatic ischemia-reperfusion.Öğe Invited response letter to the letter "Our experience regarding the association between gastrointestinal stromal tumor and bariatric surgery. A response to a letter "Gastrointestinal stromal tumor after laparoscopic sleeve gastrectomy: be awake before, during, and after a bariatric procedure"(Springer, 2019) Bilecik, Tuna; Karateke, FarukThank you for the opportunity to comment on the letter to the editor entitled BOur Experience regarding the association between Gastrointestinal Stromal Tumor and Bariatric Surgery. A response to a letter BGastrointestinal Stromal Tumor After Laparoscopic Sleeve Gastrectomy: Be Awake Before, During, and After a Bariatric Procedure.^ We read this letter with great interest and we are grateful to the authors for their interest and thoughtful comments regarding our former letter [1]. In their letter, the authors shared their experience regarding the gastrointestinal stromal tumor (GIST) as a bariatric surgery incidental finding [2]. The authors presented 12 cases which were retrospectively analyzed from a group of 50 European laparoscopic surgeons. In their case series, the demographics of the patients and anatomic tumoral localization and pathological characteristics (size, margins, CD117 marker, and mitotic index) were similar with other studies in the literature [1, 3–5].Öğe Is component separation a safe method in incisional hernias?(Istanbul Training & Research Hospital, 2019) Arslan, Ufuk; Bilecik, TunaIntroduction: Incisional hernias after abdominal surgical procedures cause morbidity, significant loss of labor and affect quality of life. In this study, we investigated whether component separation (CS) technique is a safe method in patients with incisional hernia. We evaluated the results of this technique in terms of wound infection and recurrence. Methods: Thirty incisional hernia cases operated with CS technique between February 2012 and February 2014 were included in the study. Demographic data, body mass index (BMI), American Society of Anesthesiologists (ASA) score, defect size, wound complications and recurrences after one-year follow-up were evaluated. Results: Seventeen patients were female and 13 were male. The mean age of the patients was 54.8 years (range: 29-76). The mean BMI of the patients was 26.8 kg/m(2) (range: 20.5-32.9). The mean hernia transverse diameter was 9.6 cm (range: 6.5-23). Mortality was not observed in any of the patients. Wound problems were observed in six of 30 patients in CS repair, and recurrence was observed in three of these patients. Recurrence was observed in only one of the remaining 24 patients without a wound problem. Recurrence was found to be statistically related to wound problem. There was also a significant relationship between BMI, hernia transverse diameter, ASA score and recurrence. Conclusion: In selected cases, CS technique is a safe technique for incisional hernia repair. It can be performed by paying attention to wound problems.Öğe The potential effects of ankaferd blood stopper and fibrin sealent on sleeve gastrectomy staple-line healing: An experimental study(Spandidos Publ Ltd, 2019) Sakar, Alkan; Bilecik, Tuna; Mayir, Burhan; Oruc, TahirStaple-line bleeding and leakage is a life-threatening complication in obese patients following laparoscopic sleeve gastrectomy. The aim of this study was to examine the potential effects of Ankaferd blood stopper (ABS) and Fibrin Sealant (FS; Tisseel (R)) on sleeve gastrectomy staple-line healing in an experimental animal model. A total of 30 Wistar albino female rats were divided into three groups and were subjected to sleeve gastrectomy with linear stapling. Group A (control group) had nothing administered, Group B was administered FS on the staple-line, and Group C was administered ABS on the staple-line following sleeve gastrectomy. After sacrifice on postoperative day 5, anastomotic burst pressure, tissue hydroxyproline levels and histopathological parameters were measured. The results revealed that group C had the highest mean bursting pressure level. However, the values of this parameter were not found to differ significantly between the groups (P>0.05). Group B and C had a similar hydroxyproline levels but increased compared with group A (P<0.001). Histopathological parameters were similar between the groups, except macrophage scores in group C. In the present experimental study, ABS was demonstrated to improve gastric-sleeved staple-line healing compared with FS. ABS may be used as a novel reinforcement agent in bariatric surgery.
