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Öğe The effects of different vasovasostomy techniques on motility of vas deferens (vas motility following vasovasostomy)(Springer, 2023) Pampal, Arzu; Fincan, Gokce Sevim Ozturk; Ozen, Ibrahim Onur; Isli, Fatma; Yildirim, Seniz; Ercan, Sevim; Sarioglu, YusufPurpose Vasovasostomy is used to correct vas deferens (VD) transections encountered during surgery or to reverse sterilization vasectomies. Achieving vasal patency is the primary goal and the success is assessed on various factors including VD patency, flow rates, and pregnancy rates. While preserving vas motility is not a major concern in surgical practice, it is worth noting that VD has peristaltic activity which plays crucial role during ejaculation. Any disruption in its motility could potentially lead to negative outcomes in the future. We conducted an experimental study to assess vas motility changes following vasovasostomy.Methods The study was approved by Gazi University, Animals Ethic Committee. Twenty-four rats were allocated to four groups. Left-sided VD was harvested in control group (Gr1). The rest of the animals were subjected to transection of VD. Gr2 and 3 underwent microscopic and macroscopic anastomosis, respectively, while Gr4 underwent vasal approximation. After 12 weeks, all left-sided VD were resected, electrical field stimulation (EFS) and exogenous drugs were applied to induce contractions. Statistical analyses were performed and p value < 0.05 was regarded as statistically significant.Results The first and second phases of EFS-induced contractile responses(CR) increased for Gr3 and decreased for Gr4 at submaximal and maximal frequencies. An increase only at maximal frequency for second phase EFS-induced CR was encountered for Gr2. alpha-beta-methylene-ATP-induced CR decreased for Gr3 and 4. Noradrenaline-induced CR increased for Gr2, and 3 and decreased for Gr4.Conclusion The results suggest that vasovasostomy performed using a surgical technique that minimizes disruption or damage to VD may have a favorable impact on motility.Öğe Effects of epoxygenases on the nonadrenergic noncholinergic relaxant responses induced by electrical field stimulation in rabbit corpus cavernosum(Wiley, 2019) Isli, Fatma; Yildirim, Seniz; Fincan, Gokce Sevim Ozturk; Ercan, Sevim; Sarıoğlu, YusufWe aimed to investigate the effects of epoxygenases on electrical field stimulation (EFS)-mediated nitric oxide (NO)-dependent and NO-independent nonadrenergic noncholinergic (NANC) relaxations in isolated rabbit corpus cavernosum. The tissues of 20 male adult albino rabbits (2.5-3 kg) were suspended in organ baths containing aerated Krebs solution, and isometric contractions were recorded. EFS-mediated NANC relaxations were obtained on phenylephrin (3 x 10(-5) M)-contracted tissues in the presence of guanethidine (10(-6) M) and atropine (10(-6) M). Miconazole (10(-9)-10(-4) M), 17-octadecynoic acid (ODYA) (10(-10)-10(-5) M), 14,15-epoxyeicosatrienoic acid (EET) (10(-11)-10(-8) M), 11,12-EET (10(-12)-3 x 10(-8) M) and 20-hydroxyeicosatetraenoic acid (HETE) (10(-11)-3 x 10(-8) M) were added cumulatively (n = 5-7 for each set of experiments). For NO-independent relaxations, N-omega-nitro-l-arginine methyl ester (l-NAME) (10(-4) M) was added before a group of experiments. Depending on the concentration, miconazole, 17-ODYA, 14,15-EET, 11,12-EET, and 20-HETE significantly enhanced both NO-dependent and NO-independent EFS-mediated relaxations (p < 0.05). Epoxygenases showed similar effect on NO-dependent and NO-independent relaxant responses except 20-HETE which caused significantly more enhanced relaxation on NO-dependent responses (p < 0.05). No drug caused a significant relaxation response on tissues contracted with phenylephrine. Epoxygenases contribute to EFS-mediated NO-dependent and NO-independent NANC relaxations by presynaptic mechanisms, offering a new treatment alternative for erectile dysfunction which needs to be explored in further in vivo, molecular and clinical studies.Öğe Effects of hydrogen sulfide on relaxation responses in the lower esophageal sphincter in rabbits: the potential role of potassium channels(Springer, 2024) Koc, Aysegul; Koc, Derya Sebile; Askin, Celil Ilker; Kara, Halil; Fincan, Gokce Sevim Ozturk; Ilhan, Sevil Ozger; Sarioglu, YusufHydrogen sulfide (H2S) is a significant physiologic inhibitory neurotransmitter. The main goal of this research was to examine the contribution of diverse potassium (K+) channels and nitric oxide (NO) in mediating the H2S effect on electrical field stimulation (EFS)-induced neurogenic contractile responses in the lower esophageal sphincter (LES). EFS-induced contractile responses of rabbit isolated LES strips were recorded using force transducers in organ baths that contain Krebs-Henseleit solutions (20 ml). Cumulative doses of NaHS, L-cysteine, PAG, and AOAA were evaluated in NO-dependent and NO-independent groups. The experiments were conducted again in the presence of K+ channel blockers. In both NO-dependent and NO-independent groups, NaHS, L-cysteine, PAG, and AOAA significantly reduced EFS-induced contractile responses. In the NO-dependent group, the effect of NaHS and L-cysteine decreased in the presence of 4-AP, and also the effect of NaHS decreased in the NO-dependent and independent group in the presence of TEA. In the NO-independent group, K+ channel blockers didn't change L-cysteine-induced relaxations. K+ channel blockers had no impact on the effects of PAG and AOAA. In addition, NaHS significantly relaxed 80-mM KCl-induced contractions, whereas L-cysteine, PAG, and AOAA did not. In the present study, H2S decreased the amplitudes of EFS-induced contraction responses. These results suggest that Kv channels and NO significantly contribute to exogenous H2S and endogenous H2S precursor L-cysteine inhibitory effect on lower esophageal sphincter smooth muscle.Öğe Investigation of the Contribution of Potassium Channels in the Relaxing Effect of Hydrogen Sulfide in Isolated Rabbit Lower Esophagus Sphincter(Wiley, 2023) Koc, Aysegul; Koc, Derya Sebile; Askin, Celil Ilker; Kara, Halil; Fincan, Gokce Sevim Ozturk; Ilhan, Sevil Ozger; Sarioglu, Yusuf[Abstract Not Available]Öğe Investigation of the Effects and Mechanisms of Epoxygenases in Isolated Rat Gastric Fundus(Wiley, 2023) Koc, Derya Sebil E.; Fincan, Gokce Sevim Ozturk; Askin, Celil Ilker; Kibar, Ayse Kubra; Saglam, Atiye Seda Yar; Ilhan, Sevil Ozger; Sarioglu, Yusuf[Abstract Not Available]
