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Investigation of possible associations between tryptophan/kynurenine status and FOXP3 expression in colorectal cancer
(Taylor and Francis, 2022) Ay, Ebru Nur; Hakan, Mehmet Tolgahan; Arıkan, Soykan; Demirkol, Şeyda; Doğan, Mehmet Baki; Hepokur, Ceylan Özsoy; Yaylım, İlhan; Akyüz, Filiz; Horozoğlu, Cem
Tryptophan metabolism in the tumor microenvironment exerts immunosuppressive effects by affecting the anti-tumor functions of immune cells. The immunosuppressive roles of tryptophan and tryptophan metabolites and their effects on the FOXP3 gene, highly expressed in regulatory T cells (Tregs), are remarkable. Our study aimed to investigate the relation between tryptophan metabolism and the transcription factor FOXP3 gene in colorectal cancer (CRC). Patients with CRC (n?=?159) and controls (n?=?112) were included in the study. The FOXP3 rs3761548 variant genotyping from the isolated genomic DNA was performed by PCR-RFLP. FOXP3 gene expression was determined by Q-PCR in RNAs isolated from resected tissues at the same time. Serum tryptophan, kynurenine, kynurenic acid levels of the cases were determined by HPLC. In serum samples with CRC, tryptophan level was 14.32?±?1.09??mol/L, kynurenine level was 1.33?±?0.02??mol/L, and the kynurenic acid level was 0.01?±?0.001??mol/L. The level of tryptophan was found to be low in CRC compared to control (p?