Yazar "Mat, Afife" seçeneğine göre listele

Listeleniyor 1 - 4 / 4
  • Küçük Resim Yok
    Öğe
    Antioxidant, antimicrobial, anti-urease and cytotoxic activities of various extracts from Scutellaria sibthorpii endemic to Cyprus
    (NATL INST SCIENCE COMMUNICATION-NISCAIR, 2022) Toplan, Gizem Gülsoy; Taşkın, Turgut; Öztaş, Ezgi; Hacıoğlu, Mayram; Tufan, Selin; Sarıyar, Günay; Mat, Afife
    Lamiaceae is one of the largest families in the plant kingdom. Including the genus Scutellaria whose species are used in traditional medicine in various countries for prevention and also the treatment of several disorders. In vitro biological activities of the various extracts of the aerial parts from Scutellaria sibthorpii were investigated in the present study. Antioxidant activity of the extracts was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS assays and ferric reducing antioxidant power (FRAP) assays. Cytotoxic potential and anti-urease activities of the extracts were also determined. Spectrophotometric analysis was used to establish the total phenol content of the extracts. The antibacterial activities of extracts were assessed by minimal inhibitory concentration (MIC) against seven bacteria and Candida albicans. Regarding the results, infusion showed considerable antioxidant properties in three method (IC50 value 0.133 +/- 0.0005(DPPH), 26.54 +/- 0.05 mM trolox/mg extract(ABTS), 8.76 +/- 0.1 mM Fe+2/mg extract (FRAP)). Other extracts exhibited high-to-moderate antioxidant effects. Among the studied samples, the infusion had the highest total phenolic content (0.0295 +/- 0.0002 mg GAE/mg extract). According to cytotoxicity results, n-hexane extract had strong cytotoxic effects against PC-3 and NIH/3T3 cell line with IC50 values of 330.40 mu g/mL and 340.85 mu g/mL, respectively. In the antibacterial activity screening, the ethyl acetate extract showed higher activity against Enterecocus facecalis, Klebsiella pneumoniae, Proteus mirabilis with 625 mu g/mL MIC value. According to the research carried out, it would seem that this is the first study on screening biological activities of extracts from Scutellaria sibthorpii. These findings indicate that these endemic species from Cyprus could be used in phytopharmaceutical preparation.
  • Küçük Resim
    Öğe
    Phytochemical composition and pharmacological activities of Teucrium polium L. Collected from eastern Turkey
    (TUBITAK, 2022) Gülsoy Toplan, Gizem; Göger, Fatih; Taşkın, Turgut; Ecevit-Genç, Gülay; Civaş, Ayşe; İşcan, Gökalp; Kürkçüoğlu, Mine; Mat, Afife; Başer, K. Hüsnü Can
    Teucrium species that belong to the family Lamiaceae have been traditionally used for their medicinal properties. T. polium is one the most widespread members of the genus for its use in the treatment of several diseases. In this study, the essential oil and phenolic composition of the aerial parts from T. polium were assessed by GC-FID, GC/MS, and LC-MS/MS as well as for its total phenolic content. Several extracts such as n-hexane, chloroform, methanol, and infusion were prepared and their antimicrobial, antioxidant, and also acetylcholinesterase activities were studied. According to GC/MS results, ß -caryophyllene (8.8%), t-cadinol (6.2%), (E)-nerolidol (5%), ? -cadinol (5.4%), and a-pinene (4.7%) were identified as main constituents of the essential oil. LC MS/MS analysis of the infusion and the methanol extract showed the presence of 15 phenolic compounds. Moreover, the total phenolic content of each sample was also determined and the infusion had the highest percentage of phenolics. To evaluate the antioxidant properties, the samples were tested by using DPPH” free radical scavenging, FRAP, and CUPRAC activity methods. The infusion showed the strongest radical scavenging activity, whereas n-hexane and chloroform extracts exhibited considerable reducing power effects. The MIC values for all of the examined microorganisms ranged from 15 to 2000 µg/mL with respect to antimicrobial activities. © TÜBİTAK
  • Küçük Resim
    Öğe
    Phytochemical screening and biological evaluation of Salvia hydrangea Dc. ex Benth. growing in eastern Anatolia
    (Elsevier B.V., 2022) Gülsoy Toplan, Gizem; Kürkçüoğlu, Mine; Göger, Fatih; Taşkın, Turgut; Civaş, Ayşe; İşcan, Gökalp; Ecevit Genç, Gülay; Mat, Afife; Başer, K.Hüsnü Can
    Salvia species have lately gained significant interest as a result of their suitable utilization in various industries. In the current study, S. hydrangea, one of the most consumed sages in the eastern region of Turkey, has been evaluated for phytochemical composition as well as in vitro pharmacological potential comparatively for the first time. The phytochemical composition of S.hydrangea was investigated by LC-MS/MS, GC-FID, and GC/MS. To reveal its biological activities, antioxidant, antimicrobial, and also acetylcholinesterase activities of different solvent extracts such as water, n-hexane, chloroform, and methanol were determined. According to GC/MS analysis, the primary components of the oil were identified as camphor (46.0%), 1,8-cineole (7.5%), camphene (6.8%), limonene (6.5%), a-pinene (5.6%) and b-pinene (6.1%). Additionally, in the infusion and methanol extract, rosmarinic acid and luteolin glycoside were detected as predominant phenolics by LC-MS/MS. In DPPH·, CUPRAC, and FRAP test results of the samples indicated strong to moderate antioxidant ability in all samples studied, additionally, among them, the infusion exhibited significant acetylcholine inhibition properties comparable with galanthamine. With regard to antimicrobial activity, all of the tested microorganisms had MIC values ranging from 15 to 2000 µg/mL. Based on these findings, S. hydrangea may have promising properties for a variety of industrial applications in the pharmaceutical, food, and cosmetic industries. © 2022 SAAB
  • Küçük Resim
    Öğe
    Quality of turmeric powder in herbal stores: pharmacognostical investigations on turmeric powders obtained from herbal stores in Istanbul, Turkey
    (ISTANBUL UNIV, 2021) Kuruldak, Ebru; Yilmaz, Fatmanur; Toplan, Gizem Gülsoy; Çelik, Berna Ozbek; Mat, Afife
    Background and Aims: Curcuma longa L., known as Turmeric, has been traditionally used in Asian culture since ancient times to treat several disorders. With the increase in studies on turmeric and its major compounds, it became popular. The study aims to investigate the safety and efficacy of powdered samples collected from 15 different herbal stores in Istanbul. Methods: Macroscopical, microscopical, bacteriological, and some physicochemical methods were used to evaluate the turmeric powder samples and the extracts of the samples. Additionally, the qualitative determination of curcumin in extracts was carried out by thin-layer chromatography (TLC). Results: The results of the study show that the powdered samples contain curcuminoids and are also of a moderate quality. The microbiological assay showed us the existence of high levels of pathogens. Conclusion: Turmeric powder should be consumed carefully, the storage period, and also the origin of the Turmeric is significant in consumption.