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    The first serological detection of West Nile virus infection among residents living in northern Turkey
    (Wolters Kluwer Medknow Publications, 2023) Taskin, Mehmet Hakan; Tamer, Cuneyt; Muftuoglu, Bahadir; Ozan, Emre; Kilic, Suleyman Sirri; Akkoyunlu, Gokce Kubra; Kurucay, Hanne Nur
    Background & objectives: West Nile virus (WNV) is transmitted by a mosquito-borne virus whose natural reservoir is birds. Humans and horses are considered accidental hosts. Even if the vast majority of WNV infections in humans have asymptomatic or mild disease settings, serious neurological disorders with lethal outcomes can also be observed in around 1% of the cases. We aimed to serologically investigate the presence of WNV in humans living in Black sea of Turkey, and to obtain epidemiological data that will contribute to the implementation of public health policies to control and prevent potentially other life-threatening arboviral infections.Methods: In the current study, a total of 416 human sera were collected from native patients of Samsun and its boroughs attending Samsun Training and Research Hospital; these sera were tested for WNV with pooling method, using anti-IgM and IgG ELISA commercial kits. All pools that were found positive for both IgM and IgG were individually retested for the detection of positive WNV sera. After that, all positive samples were tested using real-time PCR to detect the presence of WNV-RNA particles. Results: Total seropositivity rates of WNV in terms of IgM and IgG were found as 0.96% and 0.72%, respectively. No presence of WNV-RNA could be detected in positive samples.Interpretation & conclusion: According to the data, further studies should be conducted to better understand the epidemiological dynamics of WNV in Turkey. It is recommended that other antigenically related flaviviruses which can give cross-reaction with WNV should also be investigated.
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    INFECTIVITY AND REPLICATION INHIBITION EFFECT OF 5-FLUOROURACIL ON HERPES SIMPLEX VIRUS TYPE-1 ASSOCIATED WITH MUTATIONS IN THYMIDINE KINASE GENE
    (Soc Stiinte Farmaceutice Romania, 2022) Taskin, Mehmet Hakan; Kurucay, Hanne Nur; Kadi, Hamza; Tamer, Cuneyt; Ozan, Emre; Muftuoglu, Bahadir; Albayrak, Harun
    Two pandemics over the first two decades of the 21st century have shown that viruses with epidemic and pandemic potentials constitute a major threat to human health due to the lack of effective antivirals. Developing new antivirals and treatment strategies is also as important as vaccines play a crucial role in the first line of preventing viral infections. Using base analogues that directly target viruses and understanding their action mechanisms can present essential alternative treatment strategies to combat viral diseases. The current study investigated the effects of a base analogue on herpes simplex virus type1 (HSV-1) replication in a cell culture system using the pyrimidine analogue 5-fluorouracil (FU). After that, the full-length UL-23 gene encoding thymidine kinase (TK) of HSV-1 was sequenced to detect induced mutations. The results showed a diminishing viral titre and viral load at 2 logs and 663 times, respectively, at the end of 10 consecutive passages with 5-FU. Furthermore, two mutations substitute amino acids in the non-conserved region of the TK gene, which confers drug resistance, were also identified. The current research is a feasibility study to investigate the antiviral effects of 5-FU on DNA viruses and has reinforced the fact that 5-FU can have an antiviral effect on HSV-1. However, drug resistance for viruses should not be underestimated.