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Öğe Comparıson of cumulus cells and follıcular fluıd obtaıned from ınfertile ındıvıduals dıagnosed wıth polycystıc ovary syndrome (PCOS) and endomethrıosıs wıth samples obtaıned from healthy ındıvıduals(2023) Gürgan, Timur; Ruso, Halil; Tural, Rabia; Dinçel, Aylin Sepici; Dayanır, Duygu; Kalem, Ziya; Sarıbaş, SanemAim: Investigating the relationship between Growth differentiation factor-9 (GDF-9), Bone morphogenetic protein-15 (BMP-15) markers, apoptosis levels in cumulus cells and total oxidant (TOS)/ anti-oxidant (TAS) stress levels, inflammation parameters (interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-alpha)) in follicular fluid belonging to patients with polycystic ovary syndrome (PCOS), endometriosis (END) and male factor (MF) (control) groups. Material and Methods: GDF-9 and BMP-15 markers are determined by immunohistochemical methods, apoptosis levels are studied with TUNEL. TOS and TAS statuses are investigated with spectrophotometry, IL-6 and TNF – alpha levels are examined by Enzyme-Linked Immuno Sorbent Assay (ELISA). Results: According to the data obtained in the study; GDF-9 and BMP-15 levels are found to be lower in PCOS and END groups and apoptosis levels of cumulus cells were significantly higher at these groups . TOS levels were significantly higher in PCOS and END groups whereas follicular fluid TAS levels were not statistically significant for these groups. IL-6 and TNF – alpha levels of follicular fluid was significantly higher in PCOS. These parameters were also higher for END group, however the difference was not found to be significant. Conclusion: Our results imply that correlation between GDF-9, BMP-15 markers, apotosis levels, oxidative status, inflammation levels may be interpreted with improper environment for oocyte maturation for patients diagnosed with PCOS or END. The need for further studies on subject proceeds. However, if similar datas are obtained in further studies, it is thought that evaluation of cumulus cell properties together with especially follicular fluid oxidative stress levels will contribute to the selection of the best oocyte.Öğe Fresh versus frozen-thawed blastocyst transfer in high responders(Via Medica, 2018) Kalem, Ziya; Namlı Kalem, Müberra; Ruso, Halil; Bakirarar, Batuhan; Gurgan, TimurObjectives:This study aimed to investigate and compare the pregnancy and live birth rates in IVF cycles of frozen-thawed embryo transfers and fresh embryo transfers in a group of women with a high risk of Ovarian hyperstimulation syndrome (OHSS). Material and methods: The study group consisted of 254 women with a high level of response to controlled ovarian hyperstimulation. The patients who received fresh cycle embryo transfers with calcium infusions are referred to as the Fresh Ca+ group, and those without the calcium therapy are called the Fresh Ca- group; and we used correspondingly similar terminology for the Frozen group. Results: We observed no statistically significant differences between the cycles of fresh and frozen-thawed embryo transfers in patients with a high risk of OHSS in terms of implantation, clinical pregnancy, and live birth rates. Furthermore, these implantation, clinical pregnancy and live birth rates were not different in the cycles with or without calcium treatment. There was no statistical difference in the OHSS rates between the fresh and frozen-thawed cycles; although, the OHSS rates were less in the two calcium infusion groups (Fresh Ca+ and Frozen-thawed Ca+) than in the without-calcium group.There was no OHSS development in the subjects of the Frozen-thawed Ca+ group. Conclusions: Our study results suggest that fresh and frozen-thawed embryo transfers have similar IVF results in patients with a high risk of OHSS. Calcium infusion is beneficial in preventing OHSS without altering pregnancy rates. Both IVF protocols with calcium infusion can safely be applied in high-responder patients without lowering success rates.