Yazar "Vaziri, N." seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim
    Öğe
    Cancer-Associated Fibroblasts Regulate the Plasticity of Breast Cancer Stemness through the Production of Leukemia Inhibitory Factor
    (Multidisciplinary Digital Publishing Institute (MDPI), 2021) Vaziri, N.; Shariati, L.; Zarrabi, Ali; Farazmand, A.; Javanmard, S.H.
    Leukemia inhibitory factor (LIF), as a member of the interleukin-6 cytokine family, plays a complex role in solid tumors. However, the effect of LIF as a tumor microenvironment factor on plasticity control in breast cancer remains largely unknown. In this study, an in vitro investigation is conducted to determine the crosstalk between breast cancer cells and fibroblasts. Based on the results, cancer-associated fibroblasts are producers of LIF in the cocultivation system with breast cancer cells. Treatment with the CAF-CM and human LIF protein significantly promoted stemness through the dedifferentiation process and regaining of stem-cell-like properties. In addition, the results indicate that activation of LIFR signaling in breast cancer cells in the existence of CAF-secreted LIF can induce Nanog and Oct4 expression and increase breast cancer stem cell markers CD24?/CD44+. In contrast, suppression of the LIF receptor by human LIF receptor inhibition antibody decreased the cancer stem cell markers. We found that LIF was frequently overexpressed by CAFs and that LIF expression is necessary for dedifferentiation of breast cancer cell phenotype and regaining of cancer stem cell properties. Our results suggest that targeting LIF/LIFR signaling might be a potent therapeutic strategy for breast cancer and the prevention of tumor recurrence.
  • Küçük Resim Yok
    Öğe
    MicroRNA-200c overexpression in cancer-associated fibroblasts decreases interleukin-2 secretion
    (Blackwell Publishing, 2022) Shariati, L.; Vaseghi, G.; Vaziri, N.; Shenavar, N.; Zarrabi, A.; Haghjooy, Javanmard, S.
    miR-200c-3p is demonstrated to play the role of tumour suppressor in different tumours. However, the miR-200c-3p biological function in normal fibroblast (NF) and cancer-associated fibroblast (CAF) remains unclear. This investigation aims to study the regulatory role of miR-200c-3p in the secretion of Interleukin-2 (IL-2) in CAF and NF. CAFs and NFs were isolated from tumour and normal tissue specimens respectively. Immunocytochemistry was used to confirm the presence of a fibroblast specific marker, alpha-actin smooth muscle, in NFs and CAFs. NF and CAF were transfected with scramble and miR-200c-3p utilizing the lipofectamine 2000 reagent. The protein levels of IL-2 were measured in CAFs, NFs, and transfected groups with miR-200c-3p and scrambled using an IL-2 enzyme-linked immunoassay kit. miR-200c decreased secretion of IL-2 in transfected CAF and NF compared to controls. Results elucidated that transfection of MiR-200c-3p can decrease the IL-2 secretion and consequently reduce IL-induced tumourigenic manner in the CAF. © 2022 The Authors. Clinical and Translational Discovery published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.