Enhancing microbial diversity as well as multi-organ health in hind-limb unloaded mice

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dc.authoridSiddiqui, Ruqaiyyah/0000-0001-9646-6208
dc.authoridKhan, Naveed/0000-0001-7667-8553
dc.authorwosidSiddiqui, Ruqaiyyah/AIF-2100-2022
dc.authorwosidKhan, Naveed/AAM-2892-2021
dc.contributor.authorShama, Shama
dc.contributor.authorRanade, Anu, V
dc.contributor.authorQaisar, Rizwan
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorTauseef, Isfahan
dc.contributor.authorElmoselhi, Adel
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.date.accessioned2024-05-19T14:42:27Z
dc.date.available2024-05-19T14:42:27Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractDuring space travel, the gut microbiota is changed which can lead to health-related issues. Previously, we utilized the hind-limb unloaded (HU) mouse, which is an established ground-based in-vivo model of microgravity and observed altered gut microbiota. In this study, we evaluated the beneficial effects of novel bacterial conditioned media in HU mice to understand if they can offset the effects of unloading in the HU mouse model. We aimed to explore the influence of bacterial conditioned media on diversity and quantity of intestinal microbes in HU mice, and investigated the microarchitecture of mice retinas and kidneys to evaluate the potential systemic effects of bacterial conditioned media in HU mice. Four-month-old, male C57/Bl6 mice were separated into groups: including the ground-based control group, the HU group mice fed with vehicle as placebo (HU-placebo mice), and the HU group fed with bacterial conditioned media (HU-CP mice) and kept under controlled environmental conditions for three weeks. Next, mice were sacrificed; gut dissections were conducted, and metagenomic analysis of bacterial species was performed via DNA extraction and 16S rRNA analysis. The results revealed an HU-induced reduction in intestinal microbial diversity, and an increase in pathogenic bacteria dominated by Firmicutes (45%). In contrast, supplementation with bacterial conditioned media for three weeks led to a significant increase in gut microbial diversity with noticeable changes in the OTUs abundance in the HU mice. Additionally, HU-induced muscle weakness and structural abnormalities in the retina and kidney were partially prevented with bacterial conditioned media. Moreover, a greater diversity of several bacteria in the HUCP was observed including, Bacteriodota, Firmicutes, Proteobacteria, Actionobacteriota, Verrucomicorbiota, Cyanobacteria, Gemmatimonadota, Acidobacteriota, Chloroflexi, Myxococcota, and others. Prospective research involving molecular mechanistic studies are needed to comprehend the systemic effects of bacterial metabolites conditioned media on experimental animal models under chronic stress.en_US
dc.description.sponsorshipAir Force Office of Scientific Research (AFOSR) , USAen_US
dc.description.sponsorshipRuqaiyyah Siddiqui and Naveed Ahmed Khan are supported by the Air Force Office of Scientific Research (AFOSR) , USA.en_US
dc.identifier.doi10.1016/j.lssr.2023.08.006
dc.identifier.endpage71en_US
dc.identifier.issn2214-5524
dc.identifier.issn2214-5532
dc.identifier.pmid38245349en_US
dc.identifier.scopus2-s2.0-85170678877en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage62en_US
dc.identifier.urihttps://doi.org10.1016/j.lssr.2023.08.006
dc.identifier.urihttps://hdl.handle.net/20.500.12713/5243
dc.identifier.volume40en_US
dc.identifier.wosWOS:001169811900001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofLife Sciences In Space Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectHindlimb Unloadingen_US
dc.subjectIntestinal Microbiomeen_US
dc.subjectRetinaen_US
dc.subjectKidneysen_US
dc.subjectCrocodile Microbiomeen_US
dc.titleEnhancing microbial diversity as well as multi-organ health in hind-limb unloaded miceen_US
dc.typeArticleen_US

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