Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells

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dc.authorscopusidGamze Nur Öter / 59167977900
dc.authorwosidGamze Nur Öter / KLB-8757-2024
dc.contributor.authorÖter, Gamze Nur
dc.contributor.authorDurmuş, Ezgi
dc.contributor.authorŞen, Ali
dc.contributor.authorKoçyiğit, Abdurrahim
dc.date.accessioned2025-04-18T10:22:58Z
dc.date.available2025-04-18T10:22:58Z
dc.date.issued2024
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractObjective:Plants have been used for medicinal purposes since the beginning of human history and form the basis of modern medicine, and they are also the source of most chemotherapeutic drugs for cancer treatment. This study aims to investigate for the first time the cytotoxic and apoptotic effects of the active ethanol (RTE) and chloroform (RTC) extracts of Rubus tereticaulis leaves in the A549 non-small-cell lung cancer cell line. Materials and Methods:A549 cells were treated with RTE and RTC individually. The MTT assay was used to quantitatively detect RTE and RTC's cytotoxic effects. The fluorescent signal indicator H2DCF-DA was used to detect cellular reactive oxygen species (ROS) production. Apoptosis was evaluated by fluorescence microscope after acridine orange/ethidium bromide fluorescent staining, annexin V-FITC and immunoblotting analyses, immunofluorescence, and imaging. Results:Both RTE and RTC induced cytotoxicity in A549 cells in a dose-dependently, which was accompanied with induced ROS accumulation. Both early and late apoptotic cells detected by flow cytometry were increased in the RTE- and RTC-treated cells. In addition, the results show RTC to have higher cytotoxic and apoptotic effects and increased ROS-generation capacity than RTE. Therefore, the polarity of the solvent used to exert the anticancer effect of R. tereticaulis leaves is crucial. Conclusion:This is the first anti-cancer activity study on R. tereticaulis. The results suggest R. tereticaulis leaves to have an anti-cancer effect on lung cancer cells through ROS-mediated apoptosis and RTC to be an effective therapeutic/adjuvant strategy in cancer treatment. © 2024 Istanbul University Press. All rights reserved.
dc.identifier.citationÖter, G. N., Durmuş, E., Şen, A., & Koçyiğit, A. (2024). Induction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells. European Journal of Biology, 83(1), 50-59.
dc.identifier.doi10.26650/EurJBiol.2024.1353842
dc.identifier.endpage59
dc.identifier.issn26022575
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85195689649
dc.identifier.scopusqualityQ4
dc.identifier.startpage50
dc.identifier.urihttp://dx.doi.org/10.26650/EurJBiol.2024.1353842
dc.identifier.urihttps://hdl.handle.net/20.500.12713/7042
dc.identifier.volume83
dc.indekslendigikaynakScopus
dc.institutionauthorÖter, Gamze Nur
dc.institutionauthoridGamze Nur Öter / 0000-0002-7063-3984
dc.language.isoen
dc.publisherIstanbul University Press
dc.relation.ispartofEuropean Journal of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAnti-Cancer Effect
dc.subjectApoptosis
dc.subjectLung Cancer
dc.subjectOxidative Stress
dc.subjectRubus Tereticaulis
dc.titleInduction of Apoptosis through Oxidative Stress Caused by Rubus tereticaulis Leaves Extracts in A549 Cells
dc.typeArticle

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