Does broccoli (brassica oleracea var. italica) extract have a potential therapeutic targets on human breast cancer (MDA-MB-231) using AgNOR detection method?

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dc.authoridGözde Çobanoğlu /0000-0002-7040-6212
dc.authoridMustafa Nisari /0000-0001-7469-8921
dc.authoridMehtap Nisari /0000-0002-1126-7478
dc.authoridSümeyye Uçar /0000-0003-3378-3745
dc.authoridGülderen Kerek /0000-0002-8582-0395
dc.authoridNeriman İnanç /0000-0001-5026-4133
dc.authorscopusidGülderen Kerek /58896176800
dc.authorwosidGülderen Kerek /FCA-5916-2022
dc.contributor.authorÇobanoğlu, Gözde
dc.contributor.authorNisari, Mustafa
dc.contributor.authorNisari, Mehtap
dc.contributor.authorUçar, Sümeyye
dc.contributor.authorKerek, Gülderen
dc.contributor.authorİnanç, Neriman
dc.date.accessioned2025-04-17T08:13:54Z
dc.date.available2025-04-17T08:13:54Z
dc.date.issued30 Eylül 2023
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractIn this study, it was aimed to determine the in vitro effect of the extract obtained from mature broccoli on the breast cancer cell line. MDA-MB231 breast cancer cell line was used in the study. MDA-MB-231 cells were exposed to broccoli extract at 37°C and 5% CO2 for varying durations (24 and 48 hours) and doses (125 and 250 µl/ml).At the end of the incubation period, viability, apoptosis, cell cycle and AgNOR protein status of MDA-MB-231 cells were examined in the Muse Cell Analyzer. In the groups containing broccoli extract, a decrease in the percentage of viable cells and a significant increase in the percentage of early and total apoptosis were observed for both doses compared to the control. In the cell cycle test, the number of cells in the S phase increased in all groups.It was observed that the groups containing broccoli extract slowed down the cell cycle in the transition to the S checkpoint. AgNOR staining results also supported cell cycle and apopitosis, and AgNOR number and TAA/NA ratio decreased in the 125 µl/ml broccoli extract group after 24-48 hours and were found to be statistically significant compared to the control group. It was determined that broccoli increased apoptosis on breast cancer cells by various mechanisms and inhibited cell viability/cell growth. The results were similar to the results of AgNOR protein synthesis. The study showed that the regular and correct consumption of broccoli could be effective in preventing cancer formation and slowing its progression.
dc.identifier.citationÇobanoğlu, G., Nisari, M., Nisari, M., Uçar, S., Kerek, G., & İnanç, N. (2023). Does broccoli (brassica oleracea var. italica) extract have a potential therapeutic targets on human breast cancer (MDA-MB-231) using AgNOR detection method?. Cumhuriyet Medical Journal, 45(3), 44-56.
dc.identifier.doi10.7197/cmj.1297877
dc.identifier.endpage56
dc.identifier.issue3
dc.identifier.startpage44
dc.identifier.urihttps://doi.org/10.7197/cmj.1297877
dc.identifier.urihttps://dergipark.org.tr/tr/search?q=10.7197/cmj.1297877&section=articles
dc.identifier.urihttps://hdl.handle.net/20.500.12713/6152
dc.identifier.volume45
dc.institutionauthorKerek, Gülderen
dc.institutionauthoridGülderen Kerek /0000-0002-8582-0395
dc.language.isoen
dc.publisherSivas Cumhuriyet Üniversitesi
dc.relation.ispartofCumhuriyet Tıp Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBroccoli
dc.subjectMDA-MB-231
dc.subjectAgNOR staining
dc.titleDoes broccoli (brassica oleracea var. italica) extract have a potential therapeutic targets on human breast cancer (MDA-MB-231) using AgNOR detection method?
dc.title.alternativeBrokoli (brassica oleracea var. italica) ekstraktının insan meme kanseri hücrelerine (mda-mb-231) etkisi ve agnor tespit yöntemi ile analizi
dc.typeArticle

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