Püren Akgüner, ZeynepBal Öztürk, Ayça2021-12-302021-12-302021Akgüner, Z. P., & Bal Öztürk, A. (2020). Development of tissue scaffolds with nanoparticles for bone tissue engineering applications. Pamukkale University Journal of Engineering Sciences, 27(7), 842-850.1300-70092147-5881https://doi.org/10.5505/pajes.2020.63933https://hdl.handle.net/20.500.12713/2374In this study, a series of gelatin-methacrylate (GelMA)/ Silk fibroin tissue scaffolds with different contents of layered double hydroxides (LDHs) were prepared (J/IF LDH), the effects of Mg/Al LDH nanoparticles on physical, chemical, mechanical and biological properties of scaffolds and their use as bone tissue were investigated. Mg/Al LDH nanoparticles were synthesized by hydrothermal method and characterized by FTIR, XRD, ICP-MS, TEM, particle size and zeta potential analysis. The cytotoxicity of Mg/Al LDH nanoparticles was assesed by using 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay with osteoblast cell line. Mg/Al LDH nanoparticles were found to be non-toxic. J/ IF LDH tissue scaffolds were prepared by micro-molding technique under UV light in the presence of Irgacure2659 (photoinitiator). Their structures were characterized by FTIR analysis. Swelling analysis, mechanical strength test and cell-adhesion test were performed with osteoblast cell line. All results showed that tissue scaffolds with different contents of Mg/Al LDH nanoparticles have great potential in artificial bone tissue production for bone tissue engineering applications.Bu çalışmada, farklı oranlarda çift katmanlı hidroksit bileşikleri (LDH) içeren bir seri jelatin-metakrilat (JelMA)/ İpek fibroin (J/IF LDH) doku iskeleleri hazırlanmış, LDH nanopartiküllerinin fiziksel, kimyasal, mekaniksel ve biyolojik özellikleri üzerine etkileri ve kemik dokusu olarak kullanılabilirliği araştırılmıştır. Mg/Al LDH nanopartikülleri hidrotermal metot ile sentezlenmiş, FTIR, XRD, ICP-MS, TEM, partikül boyu ve zeta potansiyeli analizleri ile karakterize edilmiştir. Mg/Al LDH nanopartiküllerinin sitotoksisitesi osteoblast hücre hattı kullanılarak 3,(4,5-dimetiltiazol-2-il)-2,5-difenil tetrazolyum bromid (MTT) testi ile belirlenmiş ve toksik olmadıkları bulunmuştur. J/IF LDH doku iskeleleri Irgacure2659 (fotobaşlatıcı) varlığında UV ışığı altında mikro-kalıplama tekniği ile hazırlanmıştır. Yapısı FTIR analizi ile karakterize edilmiştir. Şişme analizi, mekaniksel dayanım testi ve osteoblast hücreleri ile hücre-adezyon testi gerçekleştirilmiştir. Tüm sonuçlar, Mg/Al LDH nanopartikül katkılı doku iskelelerinin kemik doku mühendisliği uygulamaları için yapay kemik üretiminde kullanılabileceğini göstermiştir.trinfo:eu-repo/semantics/openAccessTissue ScaffoldsSilk FibroinLDH NanoparticlesGelMABone Tissue EngineeringLayered DoubleHydroxidesSilk FibroinIn-VitroGelatinHydrogelsFabricationBehaviorRemovalAlDifferentiationArticle277842850WOS:000732570500010N/A10.5505/pajes.2020.63933507004