Bilecik, TunaÖzozan, Ömer VefikGökçe, Hasan2020-10-082020-10-082020Bilecik, T., Ozozan, O. V., & Gokce, H. The effect of lidocaine on TRPM 2, 6, 7 and 8 channels in liver ischemia/reperfusion model in rats.2667-663Xhttps://doi.org/10.4328/ACAM.20075https://hdl.handle.net/20.500.12713/1134Aim: The transient receptor potential melastatin (TRPM) channel is subfamilies of the transient receptor potential (TRP) channels, cation permeable channels. Ischemia and reperfusion (I / R) injury is a phenomenon highlighting cellular damage of the liver caused by oxygen. Lidocaine is a local anesthetic that blocks sodium channels and suppresses mitochondrial functions of neutrophils. In this study, we purpose to investigate the effects of TRPM2/8 and TRPM6/7 expressions after lidocaine treatment in liver ischemia/reperfusion rat model. Materials and Methods: The study was carried out on 32 male Wistar rats. The animals were randomly divided into 4 groups including sham, lidocaine, I/R group and I/R- lidocaine group. The portal vein and the hepatic artery branches were clamped for 60 minutes for complete ischemia. TRPM2/8 and TRPM6/7 gene expression levels were assessed by RT-qPCR. Results: The expression levels of TRPM2 were significantly higher in liver-I/R group compared to sham and lidocaine groups (p<0.05, p<0.000, respectively), TRPM8 genes were significantly higher in liver-I/R group compared to sham and lidocaine groups (p<0.05, p<0.05, respectively). However, the expression levels of TRPM6 gene was significantly higher in liver-I/R group compared to sham, lidocaine, and I/R-lidocaine groups (p<0.05, p<0.01, p<0.05, respectively), TRPM7 genes was significantly higher in liver-I/R group compared to sham, lidocaine and I/R-lidocaine groups (p<0.05, p<0.000, p<0.05, respectively). Discussion: In conclusion, we firstly showed that an association between the expression level of TRPM2/8, TRPM6/7, and hepatic I/R, I/R-lidocaine groups as well as TRPM2/8 and TRPM6/7 gene expressions are affected by lidocaine in the liver-I/R in a rat model.eninfo:eu-repo/semantics/openAccessSodium ChannelIschemiaReperfusionTRPMLidocaineArticle114319324WOS:000572683500016N/A10.4328/ACAM.20075