Ay, Ebru NurCaner, ArmaganHepokur, Ceylan OzsoyKalindemirtas, Ferdane DanismanEroglu, Gunes OzenKariper, I. Afsin2024-05-192024-05-1920231658-3655https://doi.org10.1080/16583655.2023.2249628https://hdl.handle.net/20.500.12713/4819The aim of this work was to investigate the antiproliferative and pro-apoptotic effects of propolis on human breast cancer cells using a simple and inexpensive nanoformulation. Various methods were used to characterize the surface and morphology of propolis nanoparticles (NP-Pro), including UV-VIS, FTIR, DLS, and EDX-SEM. The cytotoxic activity of MCF-7, MDA-MB-231 breast cancer cells was measured by XTT technique and compared with healthy cells (MCF-10A). The apoptotic effect of NP-Pro was assessed by flow cytometry using Annexin/PI. The IC50 values of NP-Pro on MCF-7, MDA-MB-231 and MCF-10A cells were 13.67 +/- 0.89, 17.89 +/- 0.6, 29.9 +/- 0.56 mu g, respectively. According to these findings, the NP-Pro form we prepared was more cytotoxic on cancer cells than propolis. Cancer cells had much stronger apoptotic activity than healthy cells (p < 0.0001). In this work, NP-Pro was produced through a distinct, straightforward, and low-cost method. In summary, the NP-Pro we synthesized may be a promising potential agent for breast cancer treatment due to its specific cytotoxicity via apoptosis.eninfo:eu-repo/semantics/openAccessBreast CancerPropolisNanoparticleCytotoxicityArticle171WOS:0010583079000012-s2.0-85169338983N/A10.1080/16583655.2023.2249628N/A