Ercin, EsinKecel-Gündüz, SerdaGök, BaharAydın, TuğbaBudama-Kılınç, YaseminKartal, Murat2022-04-062022-04-062022Ercin, E., Kecel-Gunduz, S., Gok, B., Aydin, T., Budama-Kilinc, Y., & Kartal, M. (2022). Laurus nobilis L. Essential Oil-Loaded PLGA as a Nanoformulation Candidate for Cancer Treatment. Molecules, 27(6), 1899.1420-3049https://doi.org/10.3390/molecules27061899https://hdl.handle.net/20.500.12713/2599The aim of this study was to obtain essential oil (LNEO) from the Laurus nobilis L. plant, and to prepare LNEO-loaded poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) as an approach in cancer treatment. The components of the obtained LNEO were analyzed using GC-MS. The LNEO-NPs were synthesized by the single-emulsion method. The LNEO-NPs were charac-terized using UV-Vis spectrometry, Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), and a DNA binding assay, which was performed via the UV-Vis titration method. According to the results, the LNEO-NPs had a 211.4 ± 4.031 nm average particle size, 0.068 ± 0.016 PdI, and ?7.87 ± 1.15 mV zeta potential. The encapsulation efficiency and loading capacity were calculated as 59.25% and 25.65%, respectively, and the in vitro drug release study showed an LNEO release of 93.97 ± 3.78% over the 72 h period. Moreover, the LNEO was intercalatively bound to CT-DNA. In addition, the mechanism of action of LNEO on a dual PI3K/mTOR inhibitor was predicted, and its antiproliferative activity and mechanism were determined using molecular docking analysis. It was concluded that LNEO-loaded PLGA NPs may be used for cancer treatment as a novel phytotherapeutic agent-based controlled-release system. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.eninfo:eu-repo/semantics/openAccessControlled Release SystemDNA BindingLaurel Essential OilNanoparticlePLGAArticle276WOS:0007746655000012-s2.0-85127003021Q210.3390/molecules27061899Q1