Shariati, L.Vaseghi, G.Vaziri, N.Shenavar, N.Zarrabi, A.Haghjooy, Javanmard, S.2024-05-192024-05-1920222768-0622https://doi.org/10.1002/ctd2.90https://hdl.handle.net/20.500.12713/4260miR-200c-3p is demonstrated to play the role of tumour suppressor in different tumours. However, the miR-200c-3p biological function in normal fibroblast (NF) and cancer-associated fibroblast (CAF) remains unclear. This investigation aims to study the regulatory role of miR-200c-3p in the secretion of Interleukin-2 (IL-2) in CAF and NF. CAFs and NFs were isolated from tumour and normal tissue specimens respectively. Immunocytochemistry was used to confirm the presence of a fibroblast specific marker, alpha-actin smooth muscle, in NFs and CAFs. NF and CAF were transfected with scramble and miR-200c-3p utilizing the lipofectamine 2000 reagent. The protein levels of IL-2 were measured in CAFs, NFs, and transfected groups with miR-200c-3p and scrambled using an IL-2 enzyme-linked immunoassay kit. miR-200c decreased secretion of IL-2 in transfected CAF and NF compared to controls. Results elucidated that transfection of MiR-200c-3p can decrease the IL-2 secretion and consequently reduce IL-induced tumourigenic manner in the CAF. © 2022 The Authors. Clinical and Translational Discovery published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.eninfo:eu-repo/semantics/openAccessBreast CancerCancer-Associated FibroblastMir-200c-3pNormal FibroblastArticle222-s2.0-8516548285010.1002/ctd2.90N/A