Akbar, NoorKhan, Naveed AhmedGiddey, Alexander D.Soares, Nelson C.Alharbi, Ahmad M.Alfahemi, HasanSiddiqui, Ruqaiyyah2024-05-192024-05-1920232076-2607https://doi.org10.3390/microorganisms11041072https://hdl.handle.net/20.500.12713/5195Water monitor lizards (WMLs) reside in unhygienic and challenging ecological surroundings and are routinely exposed to various pathogenic microorganisms. It is possible that their gut microbiota produces substances to counter microbial infections. Here we determine whether selected gut bacteria of water monitor lizards (WMLs) possess anti-amoebic properties using Acanthamoeba castellanii of the T4 genotype. Conditioned media (CM) were prepared from bacteria isolated from WML. The CM were tested using amoebicidal, adhesion, encystation, excystation, cell cytotoxicity and amoeba-mediated host cell cytotoxicity assays in vitro. Amoebicidal assays revealed that CM exhibited anti-amoebic effects. CM inhibited both excystation and encystation in A. castellanii. CM inhibited amoebae binding to and cytotoxicity of host cells. In contrast, CM alone showed limited toxic effects against human cells in vitro. Mass spectrometry revealed several antimicrobials, anticancer, neurotransmitters, anti-depressant and other metabolites with biological functions. Overall, these findings imply that bacteria from unusual places, such as WML gut, produce molecules with anti-acanthamoebic capabilities.eninfo:eu-repo/semantics/openAccessGut MicrobiotaAcanthamoeba CastellaniiWater Monitor LizardCytopathogenicityArticle11437110494WOS:0009793984000012-s2.0-85156272962N/A10.3390/microorganisms11041072Q2