Öztürk, ŞuleErkısa Genel, MerveOran, OranUlukaya, EnginÇelikler, SerapArı, Ferda2020-08-302020-08-302021Ozturk, S., Erkisa, M., Oran, S., Ulukaya, E., Celikler, S., & Ari, F. (2019). Lichens exerts an anti-proliferative effect on human breast and lung cancer cells through induction of apoptosis. Drug and chemical toxicology, 1-9.0148-0545https://doi.org/10.1080/01480545.2019.1573825https://hdl.handle.net/20.500.12713/317Successful cancer treatment still requires new complexes or compounds from natural sources. Therefore, we investigated anti-growth/apoptotic effects of methanol extracts of the lichen species (Xanthoparmelia somloensis (Gleyn.) Hale, Usnea intermedia (A. Massal.) Jatta, Bryoria capillaris (Ach.) Brodo & D. Hawksw and Lobaria pulmonaria (L.) Hoffm.) on human lung (A549, H1299) and breast (MCF-7, MDA-MB-231) cancer cell lines. Anti-growth effects were monitored by the MTT and ATP viability assays. Cell death mode was evaluated by employing the fluorescence staining of nucleus, caspase-cleaved cytokeratin 18 detection, caspase 3/7 activity assay, Anneksin V cytofluorimetric assay and mitochondria membrane potential assay. Among the lichen extracts, Usnea intermedia exhibited strong anti-growth activity in a dose-dependent manner (1.56–100 µg/ml) compared to the others. Usnea intermedia was especially cytotoxic against MDA-MB-231 and H1299 cells (IC 50 value for was found 3.0 and 10.2 ?g/ml respectively). The cytotoxicity was resulted from apoptosis as proved by the presence of pyknotic nuclei, caspase 3/7 activity, phosphatidylserine translocation and loss of mitochondria membrane potential. In conclusion, Usnea intermedia warrants for further in vivo evaluation as a new alternative in cancer treatment. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.eninfo:eu-repo/semantics/closedAccessApoptosisCancer Cell DeathCytotoxicityLichensUsnea IntermediaArticle30835567WOS:0006275985000042-s2.0-85062569279Q210.1080/01480545.2019.1573825Q2