Karlık, Elif2021-05-172021-05-172020Karlik, E. (2020). Display of Sukkula distributions on Barley Roots via in situ hybridization. Caryologia. International Journal of Cytology, Cytosystematics and Cytogenetics, 73(3).https://www.doi.org/10.13128/caryologia-191https://hdl.handle.net/20.500.12713/1736Retrotransposon are an abundant and ancient parts of the plant genomes that especially LTR retrotransposons influence the genome size and evolution. Sukku-la is a non-autonomous and active, relatively high copy-number retroelement. In this study, we performed fluorescence in situ hybridization (FISH) to observe the distributions of Sukkula elements (LTRs and internal-domain) by using labelled-PCR products. The localizations of Sukkula elements (LTRs and internal-domain) were observed under confocal microscope on Hordeum vulgare L. cv. Hasat root preparations. Our results revealed that Sukkula elements is still active and spread through the whole barley chro-mosomes. Additionally, the re-sequencing analysis of Sukkula LTRs demonstrated that LTRs sequences had ~65 bp gain. These analyses represent a valuable resource to reveal genome organization of barley and large sized plants.eninfo:eu-repo/semantics/closedAccessBarleyFluorescence in Situ HybridizationInternal-DomainLTRsRetrotransposonSukkulaArticle733WOS:0006410279000092-s2.0-85105012264Q410.13128/caryologia-191N/A