Coşkun, Zeynep MineErsöz, MelikeAdaş, MineHançer, Veysel SabriBoysan, Şerife NurGönen, Mustafa SaitAcar, Aynur2020-08-302020-08-302019Coskun, Z. M., Ersoz, M., Adas, M., Hancer, V. S., Boysan, S. N., Gonen, M. S., & Acar, A. (2019). Kruppel-Like Transcription Factor-4 Gene Expression and DNA Methylation Status in Type 2 Diabetes and Diabetic Nephropathy Patients. Archives of medical research, 50(3), 91-97.0188-44091873-5487https://doi.org/10.1016/j.arcmed.2019.05.012https://hdl.handle.net/20.500.12713/636Background/Aim. Diabetic nephropathy (DN) is one of the most serious microvascular complications in diabetic patients. The kruppel-like transcription factor-4 (KLF-4) affects the expression of genes involved in the pathogenesis of DN. The present study aims to identify the KLF-4 expression and DNA methylation (DNAMe) status in patients with type-2 diabetes (T2D) and DN and to reveal the contribution of the KLF-4 to the development of DN. Material and Methods. The cohort study was performed with blood samples from 120 individuals; T2D group (n = 40), DN group (n = 40) and control group (n = 40). The expression level of the KLF-4 gene was analyzed using the real-time polymerase chain reaction (qRT-PCR) and the methylation profile detected using the methylation-specific PCR (MS-PCR) technique. Results. According to our findings, KLF-4 mRNA expression in the T2D group was 1.60 fold lower than in the control group (p = 0.001). In the DN group, the expression of KLF-4 mRNA was 2.92-fold less than that of the T2D group (p = 0.001). There was no significant alteration in the DNAMe status among the groups. Conclusion. Our findings showed that regardless of the DNAMe status, KLF-4 gene expression may play a role in the development of T2D and DN. This suggests that the KLF-4 gene may be the target gene in understanding the mechanism of nephropathy, which is the most important complication of diabetes, and planning nephropathy-related treatments, but the data should be supported with more studies. (C) 2019 IMSS. Published by Elsevier Inc.eninfo:eu-repo/semantics/closedAccessDiabetic NephropathyDna MethylationEpigeneticsKruppel-Like Transcription Factor-4Type 2 DiabetesArticle503919731495395WOS:0004871682000032-s2.0-85069661283Q310.1016/j.arcmed.2019.05.012Q1