Kocak, PolenUnsal, NazCanikyan, SerliKul, YarenCohen, Steven R.Tiryaki, Tung2024-05-192024-05-1920232631-4797https://doi.org10.1093/asjof/ojad026https://hdl.handle.net/20.500.12713/5776Background: Effective cryopreservation allows for the long-term storage of living cells or tissues with the possibility of later clinical applications. Unfortunately, no successful investigations on the long-term preservation of adipose aspirates for prospective autologous fat grafting have been conducted. Objectives: In this study, we aimed to compare 3 different freezing methods to preserve adipose aspirates obtained from conventional lipoplasty to determine the optimal cryopreservation technique. Methods: To determine the optimal cryopreservation technique, hematoxylin and eosin staining, MTS assay, and Annexin assay were performed on each of the 3 groups plus a fourth control group. Group 1 served as the control, and fat tissue was analyzed immediately after adipose harvesting with no cryopreservation. For experimental Group 2, 15mL of adipose aspirates were directly frozen at -80 degrees C for up to 2 weeks. For experimental Group 3, 15mL of adipose aspirates were frozen inside the adi-frosty containing 100% isopropanol and stored at -80 degrees C for up to 2 weeks. For experimental Group 4, 15mL of adipose aspirates were frozen with freezing solution containing 90% fetal bovine serum (v/v) and 10% dimethyl sulfoxide (v/v). Results: The results demonstrated that the experimental Group 3 had significantly more live adipocytes and greater cellular function of adipose aspirates than the experimental Groups 2 and 4. Conclusion: Cryopreservation with adi-frosty containing 100% isopropanol appears to be the best means of cryopreservation of fat.eninfo:eu-repo/semantics/openAccessFat TransferSurgical-ManagementDimethyl-SulfoxideGynecomastiaGraftsTissueLiposuctionLipectomyCellsSkinComparison of Adipocyte Viability After Short-Term Cryopreservation of Adipose Aspirates Through 3 Different TechniquesArticle537180738WOS:001133595800037N/A10.1093/asjof/ojad026