Altinkaynak, CevahirTurk, MerveEkremoğlu, MuratOzdemir, Nalan2022-02-162022-02-162021Altinkaynak, C., Turk, M., Ekremoğlu, M., Özdemir, N., 2022. Peroxidase-like activity of hemoglobin-based hybrid materials against different substrates and their enhanced application for H2O2 detection. Bulletin of the Chemical Society of Ethiopia 35, 537–55.1011-39241726-801Xhttps://doi.org/10.4314/bcse.v35i3.6https://hdl.handle.net/20.500.12713/2490Organic-inorganic hybrid nanoflowers method with unique properties are preferred than conventional immobilization methods for the past decade. Hereemoglobin-based hybrid material (FlbNFs@Cu) was synthesized under different experimental conditions (pH 5.0-9.0 and 0.01-0.50 mgmL(-1) of hemoglobin) obtaining a material size of 9-10 mu m. The encapsulation percentage and weight yield of HbNFs@Cu were determined as 100% and 6.7%, respectively. The peroxidase-like activities of the material against different substrates (ABTS and Guaiacol) were compared to free hemoglobin. The HLINFs@Cu hybrid structure exhibited V-max of 3.6995 EU/mg and a Michaelis-Menten constant (K-M) of 0.1357 mM/mL. The HbNFs@Cu hybrid material was then used to catalyze the oxidation of a peroxidase substrate ABTS to the pigmented product, which provided a colorimetric and spectrophotometric detection of H2O2. The linear operating range, detectable colorimetrically as H2O2 sensor, is 0.005-0.0042 mM, while the linear operating range, detectable spectrometically, is 0.003-0.0042 mM. The limits of detection of colorimetric and spectrophotometric sensors were 0.005 mM and 0.003 mM, respectively. Collectively, these results showed that HLINFs@Cu can be used as colorimetric biosensor for H2O2 in potential applications such as pharmaceutical food, biomedical, environmental, and industrial.eninfo:eu-repo/semantics/openAccessHydrogen PeroxideHemoglobinHybrid MaterialColorimetric AssayArticle353537550WOS:0007520461000012-s2.0-85124610425Q410.4314/bcse.v35i3.6Q3