Demirel, RabiaErarpat, SezinBodur, SuleymanGunkara, Omer TahirBakirdere, SezginTurak, Fatma2024-05-192024-05-1920232365-6549https://doi.org10.1002/slct.202203851https://hdl.handle.net/20.500.12713/4751This study proposed a precise/accurate analytical method for the paracetamol (PCT) determination in human serum/urine matrices. PCT was simultaneously derivatized and preconcentrated by dispersive liquid-liquid microextraction (DLLME). Benzoyl chloride dissolved in dichloromethane was used as derivatization agent in the extraction solvent. Quadruple isotope dilution (QID) method with three calibration mixtures (A*B-x, x=1,2,3) and one sample mixture (AB) was performed to enhance the method accuracy and precision. In our research laboratory, paracetamol benzoate-d3 (PCT benzoate-d3) was synthesized and used as isotopically labelled substance (B) to conduct QID method. After the combination of QID and DLLME-GC-MS systems, percent recovery results for human serum/urine matrices were found to be 99.3-101.0 % and 98.8-99.9 %, respectively.eninfo:eu-repo/semantics/closedAccessBenzoyl ChlorideDeterminationGc-MsIsotope DilutionParacetamolArticle813WOS:0009615643000012-s2.0-85152065149N/A10.1002/slct.202203851Q2