Üstünova, SavasTakır, SelçukYılmazer, NadimBulut, HuriAltındirek, DidemNg, Özden HatırnazTansel, Cihan DemirciDoğan, Birsel Sonmez UydesÖzbek, Uğurİlkay Armutak, ElifGürevin, Ebru Gürel2020-09-102020-09-102020Ustunova, S., Takir, S., Yilmazer, N., Bulut, H., Altindirek, D., Ng, O. H., ... & Gurevin, E. G. (2020). Hydrogen Sulphide and Nitric Oxide Cooperate in Cardioprotection Against Ischemia/Reperfusion Injury in Isolated Rat Heart. In Vivo, 34(5), 2507-2516.1791-7549https://www.doi.org/10.21873/invivo.12067https://hdl.handle.net/20.500.12713/1009BACKGROUND/AIM: This study was designed to provide further evidence for the interactions between hydrogen sulfide (H2S) and nitric oxide (NO) in ischemia/reperfusion (I/R) injury. MATERIALS AND METHODS: Rat hearts were studied with the Langendorff technique using the H2S donor sodium hydrosulfide (NaHS, 40 ?M) and the cystathionine gamma-lyase (CTH or CSE) inhibitor DL-propargylglycine (PAG, 1 mM). NO synthase inhibitor L-NG-nitroarginine methyl ester (L-NAME, 30 mg/kg, 7 days) was administered before the isolation. The hearts were homogenized for biochemical and molecular analysis. RESULTS: NaHS reversed I/R-induced cardiac performance impairment, increased tissue nitric oxide production and decreased tissue markers for cardiac injury, while L-NAME inhibited these effects. The expression of CTH was increased with PAG, which was suppressed by L-NAME. CONCLUSION: H2S and NO increase each other's production suggesting their interaction and cooperation in cardioprotection against I/R injury.eninfo:eu-repo/semantics/openAccessHydrogen SulfideIschemia / Reperfusion InjuryIsolated HeartNitric OxideOxidative DamageArticle3412507251632871779WOS:0005749790000072-s2.0-85090182315Q410.21873/invivo.12067Q2