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dc.contributor.authorMilić, Mirta
dc.contributor.authorCeppi, Marcello
dc.contributor.authorBruzzone, Marco
dc.contributor.authorAzqueta, A.
dc.contributor.authorBrunborg, Gunnar
dc.contributor.authorGodschalk, Roger W.L.
dc.contributor.authorKoppen, Gudrun F.
dc.contributor.authorSardas, Semra
dc.date.accessioned2021-03-01T10:12:17Z
dc.date.available2021-03-01T10:12:17Z
dc.date.issued2021en_US
dc.identifier.citationMilić, M., Ceppi, M., Bruzzone, M., Azqueta, A., Brunborg, G., Godschalk, R., ... & Bonassi, S. (2021). The hCOMET project: international database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. Mutation Research/Reviews in Mutation Research, 108371.en_US
dc.identifier.issn1383-5742
dc.identifier.urihttps://doi.org/10.1016/j.mrrev.2021.108371
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1505
dc.description.abstractThe alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.en_US
dc.language.isoengen_US
dc.publisherElsevier B.V.en_US
dc.relation.isversionof10.1016/j.mrrev.2021.108371en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiomarkersen_US
dc.subjectComet Assayen_US
dc.subjectDNA Damageen_US
dc.subjectHuman Biomonitoringen_US
dc.subjectPooled Analysisen_US
dc.titleThe hCOMET project: international database comparison of results with the comet assay in human biomonitoring. baseline frequency of DNA damage and effect of main confoundersen_US
dc.typereviewen_US
dc.contributor.departmentİstinye Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümüen_US
dc.contributor.authorID0000-0001-5456-8636en_US
dc.contributor.institutionauthorSardas, Semra
dc.identifier.volume787en_US
dc.relation.journalMutation Research - Reviews in Mutation Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.description.wospublicationidWOS:000658539600011en_US
dc.description.pubmedpublicationid34083035en_US
dc.description.wosqualityQ1en_US


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