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New gene expression regulators: long non-coding RNAs

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Date

2019

Author

Gozukirmizi, Nermin
Karlik, Elif

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Citation

Gozukirmizi, N., & Karlik, E. (2019). New Gene Expression Regulators: Long Non-coding RNAs. In Mahat, NA and AbWahab, R and Huyop, FZ and Keyon, ASA and Attan, NB and Chandren, S (Ed.), PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): TOWARDS INNOVATIVE RESEARCH AND CROSS-DISCIPLINARY COLLABORATIONS. https://doi.org/10.1063/1.5125523

Abstract

Long non-coding RNAs (lncRNAs) play key roles in the regulation of biological processes including response to environmental stresses either transcriptional or post-transcriptional levels. The salinity tolerance of barley may play an important role in understanding the salinity mechanism. The aim of this study is the investigation of molecular responses of four barley genotypes Hordeum vulgare L. (Hasat, Beysehir 99, Konevi 98 and Tarm 92) to 150 mM salinity during 3 days of germination period. To reveal the effect of lncRNAs, we performed Real-time PCR and fluorescence in situ hybridization (FISH) studies. Study demonstrated salinity affected the expression levels of four lncRNAs, CNT0018772, CNT0031477, AK363461, and AK370506, during germination. Previous studies have shown that there was no statistical difference between barley varieties for expression level of CNT0018772 (p>0.05). However, our study indicated that there was statistically significant difference between barley varieties for CNT0031477 (p<0.05). Additionally, there was statistically significant difference between barley varieties for the two barley lncRNAs of AK363461 and AK370506, (p<0.05). We were also able to observe the localization of these four lncRNAs on barley chromosomes, which were exhibited under confocal microscope via in situ hybridization on barley root preparations. This study further supports the reasons why nowadays, lncRNAs are considered as major regulators.

Source

Proceedings of The 2nd International Conference On Biosciences and Medical Engineering (Icbme2019): Towards Innovative Research and Cross-Disciplinary Collaborations

Volume

2155

URI

https://doi.org/10.1063/1.5125523
https://hdl.handle.net/20.500.12713/673

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  • Eczane Hizmetleri Programı Bildiri Koleksiyonu [1]
  • Eczane Hizmetleri Programı Makale Koleksiyonu [11]
  • Moleküler Biyoloji ve Genetik Bölümü Bildiri Koleksiyonu [5]
  • Scopus İndeksli Yayınlar Koleksiyonu [1892]
  • WoS İndeksli Yayınlar Koleksiyonu [1965]



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