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dc.contributor.authorTombulturk, Fatma Kubraen_US
dc.contributor.authorKasap, M.en_US
dc.contributor.authorTuncdemir, M.en_US
dc.contributor.authorPolat, E.en_US
dc.contributor.authorSirekbasan, S.en_US
dc.contributor.authorKanli, A.en_US
dc.contributor.authorKanigur-Sultuybek, G.en_US
dc.date.accessioned2020-08-30T20:07:36Z
dc.date.available2020-08-30T20:07:36Z
dc.date.issued2018
dc.identifier.citationTombulturk, F. K., Kasap, M. U. R. A. T., Tuncdemir, M., Polat, E., Sirekbasan, S., Kanli, A. Y. L. İ. N., & Kanigur-Sultuybek, G. (2018). Effects of Lucilia sericata on wound healing in streptozotocin-induced diabetic rats and analysis of its secretome at the proteome level. Human & experimental toxicology, 37(5), 508-520.en_US
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.urihttps://doi.org/10.1177/0960327117714041
dc.identifier.urihttps://hdl.handle.net/20.500.12713/796
dc.descriptionTombulturk, Fatma Kubra/0000-0002-4358-2309; Kasap, Murat/0000-0001-8527-2096; Tuncdemir, Matem/0000-0002-5300-4449; Sirekbasan, Serhat/0000-0001-7967-3539; Tombulturk, Fatma Kubra/0000-0002-4358-2309en_US
dc.descriptionWOS: 000429812900007en_US
dc.descriptionPubMed: 28621210en_US
dc.descriptionTombulturk, Fatma Kubra (isu author)en_US
dc.description.abstractThe use of Lucilia sericata larvae on the healing of wounds in diabetics has been reported. However, the role of the excretion/secretion (ES) products of the larvae in treatment of diabetic wounds remains unknown. This study investigated whether application of the ES products of L. sericata on the wound surface could improve the impaired wound healing in streptozotocin-induced diabetic rats. Additional analysis was performed to understand proteome content of L. sericata secretome to understand ES contribution at the molecular level. For this purpose, full-thickness skin wounds were created on the backs of diabetic and control rats. A study was conducted to assess the levels of the ES-induced collagen I/III expression and to assay nuclear factor B (NF-B) (p65) activity in wound biopsies and ES-treated wounds of diabetic rat skin in comparison to the controls. The expression levels of collagen I/III and NF-B (p65) activity were determined at days 3, 7, and 14 after wounding using immunohistological analyses and enzyme-linked immunosorbent assay technique. The results indicated that treatment with the ES extract increased collagen I expressions of the wound control and diabetic tissue. But the increase in collagen I expression in the controls was higher than the one in the diabetics. NF-B (p65) activity was also increased in diabetic wounds compared to the controls, whereas it was decreased in third and seventh days upon ES treatment. The results indicated that ES products of L. sericata may enhance the process of wound healing by influencing phases such as inflammation, NF-B (p65) activity, collagen synthesis, and wound contraction. These findings may provide new insights into understanding of therapeutic potential of ES in wound healing in diabetics.en_US
dc.description.sponsorshipResearch Fund of The University of IstanbulIstanbul University [35048]en_US
dc.description.sponsorshipThis study was supported by the Research Fund of The University of Istanbul (Project Number 35048). We appreciate this support.en_US
dc.language.isoengen_US
dc.publisherSage Publications Ltden_US
dc.relation.isversionof10.1177/0960327117714041en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNuclear Factor -Ben_US
dc.subjectCollagenen_US
dc.subjectStz Diabetesen_US
dc.subjectMaggot Debridement Therapyen_US
dc.subjectLucilia Sericataen_US
dc.titleEffects of Lucilia sericata on wound healing in streptozotocin-induced diabetic rats and analysis of its secretome at the proteome levelen_US
dc.typearticleen_US
dc.contributor.departmentİstinye Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Bölümüen_US
dc.contributor.authorID0000-0002-4358-2309en_US
dc.contributor.institutionauthorTombulturk, Fatma Kubraen_US
dc.identifier.volume37en_US
dc.identifier.issue5en_US
dc.identifier.startpage508en_US
dc.identifier.endpage520en_US
dc.relation.journalHuman & Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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