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dc.contributor.authorKaraöz, Erdal
dc.contributor.authorDemircan, Pinar Cetinalp
dc.contributor.authorErman, Gulay
dc.contributor.authorSun Gungorurler, Eda
dc.contributor.authorSariboyaci, Ayla Eker
dc.date.accessioned2020-08-30T20:08:05Z
dc.date.available2020-08-30T20:08:05Z
dc.date.issued2017
dc.identifier.citationKaraöz, E., Demircan, P. Ç., Erman, G., Güngörürler, E., & Sarıboyacı, A. E. (2017). Comparative analyses of immunosuppressive characteristics of bone-marrow, Wharton’s jelly, and adipose tissue-derived human mesenchymal stem cells. Turkish Journal of Hematology, 34(3), 213.en_US
dc.identifier.issn1300-7777
dc.identifier.issn1308-5263
dc.identifier.urihttps://doi.org/10.4274/tjh.2016.0171
dc.identifier.urihttps://hdl.handle.net/20.500.12713/881
dc.descriptionSariboyaci, Ayla Eker/0000-0003-4536-9859; Sun, Eda/0000-0003-0320-5784; ERMAN, GULAY/0000-0002-9926-369Xen_US
dc.descriptionWOS: 000410936000003en_US
dc.descriptionPubMed: 27610554en_US
dc.description.abstractObjective: Mesenchymal stem cells (MSCs), which possess immunosuppressive characteristics on induced T-cells, were shown to be applicable in prevention and treatment of graft-versus-host disease. However, knowledge of effective cell sources is still limited. In this study, MSCs from different human tissues, i.e. bone marrow (BM), Wharton's jelly (WJ), and adipose tissue, were isolated, and the immune suppression of stimulated T cells was analyzed comparatively. Materials and Methods: MSCs were co-cultured with phytohemagglutinin-induced T-cells with co-culture techniques with and without cell-to-cell contact. After co-culture for 24 and 96 h, the proliferation rate of T cells was estimated by carboxyfluorescein succinimidyl ester and apoptosis by annexin V/PI methods. Both T cells and MSCs were analyzed with respect to gene expressions by real-time polymerase chain reaction and their specific protein levels by ELISA. Results: The results showed that all three MSC lines significantly suppressed T-cell proliferation; BM-MSCs were most effective. Similarly, T-cell apoptosis was induced most strongly by BM-MSCs in indirect culture. In T cells, the genes in NFkB and tumor necrosis factor pathways were silenced and the caspase pathway was induced after co-culture. These results were confirmed with the measurement of protein levels, like transforming growth factor beta 1, IL-6, interferon-gamma, interleukin (IL)-2, and tumor necrosis factor-alpha. Additionally, IL-17A was detected in high levels in WJ-MSC co-cultures. We showed that IL-17A-producing Tregs are the key mediators in the treatment of graft-versus-host disease. Conclusion: BM-MSCs, which have been used in clinical applications for a while, showed the greatest immunosuppressive effect compared to other MSCs. However, a promising cell source could also be WJ, which is also effective in suppression with fewer ethical concerns. We described the molecular mechanism of WJ-MSCs in allogenic transplants for the first time.en_US
dc.description.sponsorshipTurkish Society of Hematologyen_US
dc.description.sponsorshipThis work was supported by the Turkish Society of Hematology. We thank Zehra Seda Unal and Alparslan Okcu for their excellent technical assistance.en_US
dc.language.isoengen_US
dc.publisherGalenos Yayinciliken_US
dc.relation.isversionof10.4274/tjh.2016.0171en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectImmunoregulatory Effecten_US
dc.subjectCo-Cultureen_US
dc.subjectMesenchymal Stem Cellsen_US
dc.subjectT Cellsen_US
dc.titleComparative analyses of immunosuppressive characteristics of bone-marrow, wharton's jelly, and adipose tissue-derived human mesenchymal stem cellsen_US
dc.typearticleen_US
dc.contributor.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.contributor.authorID0000-0002-9992-833Xen_US
dc.contributor.authorID0000-003-0320-5784en_US
dc.contributor.institutionauthorKaraoz, Erdalen_US
dc.contributor.institutionauthorSun Gungorurler, Edaen_US
dc.identifier.volume34en_US
dc.identifier.issue3en_US
dc.identifier.startpage213en_US
dc.identifier.endpage225en_US
dc.relation.journalTurkish Journal of Hematologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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