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    Boric acid’s cytotoxic effect on CD133+ cancer stem cells in H460 cell line
    (2023) Acar, Muradiye
    Aim: Cancer stem cells (CSCs) are found in a wide range of cancers, and CD133 is one of the most commonly used markers for detecting CSCs. Compared to CD133- populations in lung cancer, CD133+ cells are more capable of self-renewal, drug resistance and tumor initiation and demonstrate increased CSC capabilities. Though the anti-cancer activity of boric acid (BA) has been reported in relation to various cancers, there is no study showing its effect on CSCs. The aim of this study is to investigate the anti-cancer effects of BA in CD133+ CSCs and CD133- cells from lung cancer cells. Materials and Methods: The H460 lung carcinoma cell strain was obtained from the American Type Culture Collection. CD133+ CSCs and CD133- cells were separated using CD133 antibody conjugated to magnetic beads. The sphere formation assay (coated with SpheroMake) was used to assess the ability to maintain stemness. Cells were grown in serum-free RPMI1640 medium (with B27, N2 supplement, EGF and bFGF) to observe the development of spheres. To determine CD133 mRNA expression, total RNA was extracted and RT-qPCR was performed. The xCELLigence Real-Time Cellular Analysis (RTCA) system was used to determine the cytotoxic effects of BA. 25 mM, 12 mM, 6 mM, 3 mM, and 1 mM BA solutions were added to CD133+ CSCs and CD133- cells. Results: The CD133+ CSCs composed approximately 1% of H460 cells. The mRNA expression of CD133 was 16 times higher in CD133+ CSCs cells than in CD133- cells. CD133- cells failed to form spheres but CD133+ CSCs did so, demonstrating CD133+ CSCs stemness characteristics. The xCELLigence RTCA system determined the IC50 doses of BA to be 6.7 mM and 5 mM for CD133+ CSCs and CD133- cells. Conclusion: BA exhibited anti-cancer activity against the CD133+ CSCs and CD133- cells. This study is the first to demonstrate BA’s anti-cancer effect on CSCs.
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    Mediterranean Journal of Hematology and Infectious Diseases
    (Mattioli 1885, 2023) Qipa, Egzona; Acar, Muradiye; Bozkurt, Sureyya; Buyukdogan, Murat; Sonmez, Hazal B.; Sayitoglu, Muge; Erbilgin, Yucel
    Acute lymphoblastic leukemia (ALL) is a malignant disease of hematopoietic stem cells. B cell ALL (B-ALL) is characterized by highly proliferative and poorly differentiated progenitor B cells in the bone marrow. Chromosomal rearrangements, aberrant cell signaling, and mutations lead to dysregulated cell cycle and clonal proliferation of abnormal B cell progenitors. In this study, we aimed to examine hot spot genetic variations in the RUNX1, IDH2, and IL2RA genes in a group of (n=52) pediatric B-ALL. Sanger sequencing results revealed a rare RUNX1 variant p.Leu148Gln in one B-ALL patient with disease recurrence. Additionally, common intronic variations rs12358961 and rs11256369 of IL2RA were determined in two patients. None of the patients had the IDH2 variant.RUNX1, IDH2, and IL2RA variations were rare events in ALL. This study detected a novel pathogenic RUNX1 variation in a patient with a poor prognosis. Examining prognostically important genetic anomalies of childhood lymphoblastic leukemia patients and the signaling pathway components will pilot more accurate prognosis estimations.
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    A novel ganciclovir resistance mutation in the UL97 gene of the HHV-5 in an adult hematopoietic stem cell transplant recipient
    (Future Medicine Ltd, 2017) Hançer, Veysel Sabri; Sağlam Yarımcan, Filiz; Büyükdoğan, Murat; Akı, Şahika Zeynep; Öksüz, Burcu; Acar, Kadir; Acar, Muradiye; Bulut, Pelin
    Therapeutic management of cytomegalovirus (CMV) disease in hematopoietic stem cell transplantation patients can become a challenge because of the emergence of anti-CMV drug resistance. This case report presents a patient with clinical ganciclovir resistance due to a new mutation: histidine-to-asparagine change at residue 393 of UL97. This mutation, which is located in the nonfunctional region of the UL97 gene, is very unusual. Having more information about the mutations leading to drug resistance in CMV is important for both improved clinical management and development of new diagnostic tests and drugs.
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    Prevalence of human papilloma virus types in Turkish and Albanian women
    (Wolters Kluwer Medknow Publications, 2018) Hançer, Veysel Sabri; Büyükdoğan, Murat; Bylykbashi, Ilta; Öksüz, Burcu; Acar, Muradiye
    Background: Human papilloma virus (HPV) infection is the major etiologic agent of cervical carcinoma. The aim of this study was to determine the prevalence of HPV infection and genotype distribution in cervical swabs from 2,234 Turkish and 357 Albanian women with similar lifestyles from two different countries. Materials and Methods: HPV detection and typing were performed by type specific multiplex fluorescent PCR and fragments were directly genotyped by high resolution fluorescence capillary electrophoresis. Results: The most common type was HPV 16 and the second one was HPV 6 for both country. The third common type was 39 and 18 for Turkish and Albanian women, respectively. Conclusions: When we compare our results with other studies, there are differences between the frequency and order of the HPV genotypes detected at the second and subsequent frequencies. This may due to differences in the quality and type of samples analyzed, as well as the HPV detection methods.