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  • Küçük Resim Yok
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    A novel wound healing agent with antimicrobial properties: BSA-inorganic hybrid nanoflowers
    (WILEY, 2022) Ekremoğlu, Murat; Haciosmanoglu, E.; Hacioglu, M.; Altinkaynak, C.; Ozdemir, N.
    No Abstract Available.
  • Küçük Resim
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    Peroxidase-like activity of hemoglobin-based hybrid materials against different substrates and their enhanced application for H2O2 detection
    (CHEM SOC ETHIOPIA, 2021) Altinkaynak, Cevahir; Turk, Merve; Ekremoğlu, Murat; Ozdemir, Nalan
    Organic-inorganic hybrid nanoflowers method with unique properties are preferred than conventional immobilization methods for the past decade. Hereemoglobin-based hybrid material (FlbNFs@Cu) was synthesized under different experimental conditions (pH 5.0-9.0 and 0.01-0.50 mgmL(-1) of hemoglobin) obtaining a material size of 9-10 mu m. The encapsulation percentage and weight yield of HbNFs@Cu were determined as 100% and 6.7%, respectively. The peroxidase-like activities of the material against different substrates (ABTS and Guaiacol) were compared to free hemoglobin. The HLINFs@Cu hybrid structure exhibited V-max of 3.6995 EU/mg and a Michaelis-Menten constant (K-M) of 0.1357 mM/mL. The HbNFs@Cu hybrid material was then used to catalyze the oxidation of a peroxidase substrate ABTS to the pigmented product, which provided a colorimetric and spectrophotometric detection of H2O2. The linear operating range, detectable colorimetrically as H2O2 sensor, is 0.005-0.0042 mM, while the linear operating range, detectable spectrometically, is 0.003-0.0042 mM. The limits of detection of colorimetric and spectrophotometric sensors were 0.005 mM and 0.003 mM, respectively. Collectively, these results showed that HLINFs@Cu can be used as colorimetric biosensor for H2O2 in potential applications such as pharmaceutical food, biomedical, environmental, and industrial.