Peroxidase-like activity of hemoglobin-based hybrid materials against different substrates and their enhanced application for H2O2 detection
Yükleniyor...
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
CHEM SOC ETHIOPIA
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Organic-inorganic hybrid nanoflowers method with unique properties are preferred than conventional immobilization methods for the past decade. Hereemoglobin-based hybrid material (FlbNFs@Cu) was synthesized under different experimental conditions (pH 5.0-9.0 and 0.01-0.50 mgmL(-1) of hemoglobin) obtaining a material size of 9-10 mu m. The encapsulation percentage and weight yield of HbNFs@Cu were determined as 100% and 6.7%, respectively. The peroxidase-like activities of the material against different substrates (ABTS and Guaiacol) were compared to free hemoglobin. The HLINFs@Cu hybrid structure exhibited V-max of 3.6995 EU/mg and a Michaelis-Menten constant (K-M) of 0.1357 mM/mL. The HbNFs@Cu hybrid material was then used to catalyze the oxidation of a peroxidase substrate ABTS to the pigmented product, which provided a colorimetric and spectrophotometric detection of H2O2. The linear operating range, detectable colorimetrically as H2O2 sensor, is 0.005-0.0042 mM, while the linear operating range, detectable spectrometically, is 0.003-0.0042 mM. The limits of detection of colorimetric and spectrophotometric sensors were 0.005 mM and 0.003 mM, respectively. Collectively, these results showed that HLINFs@Cu can be used as colorimetric biosensor for H2O2 in potential applications such as pharmaceutical food, biomedical, environmental, and industrial.
Açıklama
Anahtar Kelimeler
Hydrogen Peroxide, Hemoglobin, Hybrid Material, Colorimetric Assay
Kaynak
BULLETIN OF THE CHEMICAL SOCIETY OF ETHIOPIA
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
35
Sayı
3
Künye
Altinkaynak, C., Turk, M., Ekremoğlu, M., Özdemir, N., 2022. Peroxidase-like activity of hemoglobin-based hybrid materials against different substrates and their enhanced application for H2O2 detection. Bulletin of the Chemical Society of Ethiopia 35, 537–55.
