Peroxidase-like activity of hemoglobin-based hybrid materials against different substrates and their enhanced application for H2O2 detection

Basit Öğe Kaydı
dc.authoridMurat Ekremoğlu / 0000-0002-8355-7052
dc.authorscopusidMurat Ekremoğlu / 16202274000
dc.authorwosidMurat Ekremoğlu / AGI-0385-2022
dc.contributor.authorAltinkaynak, Cevahir
dc.contributor.authorTurk, Merve
dc.contributor.authorEkremoğlu, Murat
dc.contributor.authorOzdemir, Nalan
dc.date.accessioned2022-02-16T06:10:55Z
dc.date.available2022-02-16T06:10:55Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractOrganic-inorganic hybrid nanoflowers method with unique properties are preferred than conventional immobilization methods for the past decade. Hereemoglobin-based hybrid material (FlbNFs@Cu) was synthesized under different experimental conditions (pH 5.0-9.0 and 0.01-0.50 mgmL(-1) of hemoglobin) obtaining a material size of 9-10 mu m. The encapsulation percentage and weight yield of HbNFs@Cu were determined as 100% and 6.7%, respectively. The peroxidase-like activities of the material against different substrates (ABTS and Guaiacol) were compared to free hemoglobin. The HLINFs@Cu hybrid structure exhibited V-max of 3.6995 EU/mg and a Michaelis-Menten constant (K-M) of 0.1357 mM/mL. The HbNFs@Cu hybrid material was then used to catalyze the oxidation of a peroxidase substrate ABTS to the pigmented product, which provided a colorimetric and spectrophotometric detection of H2O2. The linear operating range, detectable colorimetrically as H2O2 sensor, is 0.005-0.0042 mM, while the linear operating range, detectable spectrometically, is 0.003-0.0042 mM. The limits of detection of colorimetric and spectrophotometric sensors were 0.005 mM and 0.003 mM, respectively. Collectively, these results showed that HLINFs@Cu can be used as colorimetric biosensor for H2O2 in potential applications such as pharmaceutical food, biomedical, environmental, and industrial.en_US
dc.identifier.citationAltinkaynak, C., Turk, M., Ekremoğlu, M., Özdemir, N., 2022. Peroxidase-like activity of hemoglobin-based hybrid materials against different substrates and their enhanced application for H2O2 detection. Bulletin of the Chemical Society of Ethiopia 35, 537–55.en_US
dc.identifier.doi10.4314/bcse.v35i3.6en_US
dc.identifier.endpage550en_US
dc.identifier.issn1011-3924en_US
dc.identifier.issn1726-801Xen_US
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85124610425en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage537en_US
dc.identifier.urihttps://doi.org/10.4314/bcse.v35i3.6
dc.identifier.urihttps://hdl.handle.net/20.500.12713/2490
dc.identifier.volume35en_US
dc.identifier.wosWOS:000752046100001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorEkremoğlu, Murat
dc.language.isoenen_US
dc.publisherCHEM SOC ETHIOPIAen_US
dc.relation.ispartofBULLETIN OF THE CHEMICAL SOCIETY OF ETHIOPIAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHydrogen Peroxideen_US
dc.subjectHemoglobinen_US
dc.subjectHybrid Materialen_US
dc.subjectColorimetric Assayen_US
dc.titlePeroxidase-like activity of hemoglobin-based hybrid materials against different substrates and their enhanced application for H2O2 detectionen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Altinkaynak-2022-Peroxidase-like-activity-of-hemoglo.pdf
Boyut:
614.96 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu