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  • Küçük Resim
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    Evaluation of anticancer effects of carboplatin-gelatin nanoparticles in different sizes synthesized with newly self-assembly method by exposure to IR light
    (2022) Danışman Kalındemirtaş, Ferdane; Afşin Kariper, İshak; Erdemir, Gökçe; Sert, Esra; Erdem Kuruca, Serap
    Carboplatin (CP), a platinum analog, is one of the most widely used chemotherapeutic agents in the treatment of colorectal cancer. Although platinum-based drugs are quite efective in anticancer treatments, their use in a wide spectrum and efective treatment possibilities are limited due to their systemic side efects and drug resistance development. In recent years, studies have focused on increasing the therapeutic efcacy of platinum-based drugs with drug delivery systems. Gelatin, a protein, obtained by the hydrolysis of collagen, is a biocompatible and biodegradable material that can be used in nano drug delivery systems. In this study, CP-loaded gelatin-based NPs (CP-NPs) were exposed to IR light in diferent temperatures at 30, 35, 40, 45, and 50 °C and characterized by FESEM-EDX, FTIR, UV–Vis, DLS. Accordingly, we synthesized gelatin-based CP-NPs of diferent sizes between 10–290 nm by exposure to IR. We found that CP-NPs-50, 16 nm nano-sized, obtained at 50 °C had the most cytotoxicity and was 2.2 times more efective than the free drug in HCT 116 colon cancer cells. Moreover, we showed that the cytotoxicity of CP-NPs-50 in normal HUVEC cells was lower. Additionally, we demonstrated that CP-NPs enhanced apoptotic activity while not developing MDR1-related resistance in colon cancer cells. In this study, for the frst time drug loaded gelatin-based nanoparticles were synthesized in diferent sizes with a newly self-assembly method by exposing them to infrared light at diferent temperatures and their anticancer efects were evaluated subsequently.
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    Spectrochemical and biochemical assay comparison study of the healing effect of the aloe vera and hypericum perforatum loaded nanofiber dressings on diabetic wound
    (Elsevier, 2021) Güleken, Zozan; Depciuch, Joanna; Ege, Hasan; İlbay, Gül; Kalkandelen, Cevriye; Özbeyli, Dilek; Bulut, Huri; Tarhan, Nevzat; Erdem Kuruca, Serap
    Diabetic wounds have a slow healing process and easy to be infected. In addition to current drug treatments, supportive approaches are needed for diabetic wound treatment. In this study, we aimed to load Aloe Vera (AV) and Hypericum perforatum oil (HPO) with PCL/Ge (Poly (?-caprolactone)/Gelatine) polymeric biodegradable by electrospinning method into nanofiber dressings on an experimental diabetic wound model to compare the diabetic wound healing effect. Changes in the amount and chemical structure of phospholipids, proteins, and lipids were investigated in the blood and serum samples of the animals using Fourier transform infrared (FTIR) analysis. To evaluate biological events associated with the wound repair process in inflammatory phase we used oxidant and antioxidant status to determine the healing status of wounds such as Total antioxidant status (TAS), Total oxidant level (TOS) and tumor necrosis factor alpha (TNF-?) levels. TOS level increased in DM groups and decreased in the AV and HPO group. Oxidative stress index decreased and TNF-? level increased in the HPO group. FTIR spectra showed changes in the phospholipids, proteins, and carbon chain of lipids in the whole blood as well as serum of DM rats. FTIR spectra combined with Principal component analysis (PCA) showed, that treated DM rats by AV and HPO caused return chemical structure of blood and serum to this observed in control group. Higher similarity with control group for HPO rats was observed. HPO is better than AV in the alternative for healing on diabetic wound. Thus, we have demonstrated that IR spectroscopy and multivariate data analysis and biochemical assays are consistent and correlative with each other.