Spectrochemical and biochemical assay comparison study of the healing effect of the aloe vera and hypericum perforatum loaded nanofiber dressings on diabetic wound
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Diabetic wounds have a slow healing process and easy to be infected. In addition to current drug treatments, supportive approaches are needed for diabetic wound treatment. In this study, we aimed to load Aloe Vera (AV) and Hypericum perforatum oil (HPO) with PCL/Ge (Poly (?-caprolactone)/Gelatine) polymeric biodegradable by electrospinning method into nanofiber dressings on an experimental diabetic wound model to compare the diabetic wound healing effect. Changes in the amount and chemical structure of phospholipids, proteins, and lipids were investigated in the blood and serum samples of the animals using Fourier transform infrared (FTIR) analysis. To evaluate biological events associated with the wound repair process in inflammatory phase we used oxidant and antioxidant status to determine the healing status of wounds such as Total antioxidant status (TAS), Total oxidant level (TOS) and tumor necrosis factor alpha (TNF-?) levels. TOS level increased in DM groups and decreased in the AV and HPO group. Oxidative stress index decreased and TNF-? level increased in the HPO group. FTIR spectra showed changes in the phospholipids, proteins, and carbon chain of lipids in the whole blood as well as serum of DM rats. FTIR spectra combined with Principal component analysis (PCA) showed, that treated DM rats by AV and HPO caused return chemical structure of blood and serum to this observed in control group. Higher similarity with control group for HPO rats was observed. HPO is better than AV in the alternative for healing on diabetic wound. Thus, we have demonstrated that IR spectroscopy and multivariate data analysis and biochemical assays are consistent and correlative with each other.
Açıklama
Anahtar Kelimeler
Aloe Vera Extract, Biochemical Assays, Diabetic Wound Healing, FTIR Analysis, Hypericum Perforatum Oil, Inflammation, Polymeric Nanofibers Dressings
Kaynak
Spectrochim Acta A Mol Biomol Spectrosc .
WoS Q Değeri
Q1
Scopus Q Değeri
N/A
Cilt
Sayı
254
Künye
Guleken, Z., Depciuch, J., Ege, H., İlbay, G., Kalkandelen, C., Ozbeyli, D., Bulut, H., Sener, G., Tarhan, N., & Erdem Kuruca, S. (2021). Spectrochemical and biochemical assay comparison study of the healing effect of the Aloe vera and Hypericum perforatum loaded nanofiber dressings on diabetic wound. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 254, 119639. Advance online publication. https://doi.org/10.1016/j.saa.2021.119639
