Spectrochemical and biochemical assay comparison study of the healing effect of the aloe vera and hypericum perforatum loaded nanofiber dressings on diabetic wound

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dc.authoridHuri Bulut / 0000-0003-2706-9625en_US
dc.authorscopusidHuri Bulut / 57185264300
dc.authorwosidHuri Bulut / AAM-1432-2020
dc.contributor.authorGüleken, Zozan
dc.contributor.authorDepciuch, Joanna
dc.contributor.authorEge, Hasan
dc.contributor.authorİlbay, Gül
dc.contributor.authorKalkandelen, Cevriye
dc.contributor.authorÖzbeyli, Dilek
dc.contributor.authorBulut, Huri
dc.contributor.authorTarhan, Nevzat
dc.contributor.authorErdem Kuruca, Serap
dc.date.accessioned2021-03-22T12:10:14Z
dc.date.available2021-03-22T12:10:14Z
dc.date.issued2021en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractDiabetic wounds have a slow healing process and easy to be infected. In addition to current drug treatments, supportive approaches are needed for diabetic wound treatment. In this study, we aimed to load Aloe Vera (AV) and Hypericum perforatum oil (HPO) with PCL/Ge (Poly (?-caprolactone)/Gelatine) polymeric biodegradable by electrospinning method into nanofiber dressings on an experimental diabetic wound model to compare the diabetic wound healing effect. Changes in the amount and chemical structure of phospholipids, proteins, and lipids were investigated in the blood and serum samples of the animals using Fourier transform infrared (FTIR) analysis. To evaluate biological events associated with the wound repair process in inflammatory phase we used oxidant and antioxidant status to determine the healing status of wounds such as Total antioxidant status (TAS), Total oxidant level (TOS) and tumor necrosis factor alpha (TNF-?) levels. TOS level increased in DM groups and decreased in the AV and HPO group. Oxidative stress index decreased and TNF-? level increased in the HPO group. FTIR spectra showed changes in the phospholipids, proteins, and carbon chain of lipids in the whole blood as well as serum of DM rats. FTIR spectra combined with Principal component analysis (PCA) showed, that treated DM rats by AV and HPO caused return chemical structure of blood and serum to this observed in control group. Higher similarity with control group for HPO rats was observed. HPO is better than AV in the alternative for healing on diabetic wound. Thus, we have demonstrated that IR spectroscopy and multivariate data analysis and biochemical assays are consistent and correlative with each other.en_US
dc.identifier.citationGuleken, Z., Depciuch, J., Ege, H., İlbay, G., Kalkandelen, C., Ozbeyli, D., Bulut, H., Sener, G., Tarhan, N., & Erdem Kuruca, S. (2021). Spectrochemical and biochemical assay comparison study of the healing effect of the Aloe vera and Hypericum perforatum loaded nanofiber dressings on diabetic wound. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 254, 119639. Advance online publication. https://doi.org/10.1016/j.saa.2021.119639en_US
dc.identifier.doi10.1016/j.saa.2021.119639en_US
dc.identifier.issue254en_US
dc.identifier.pmid33743307en_US
dc.identifier.scopus2-s2.0-85102651288en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage119639en_US
dc.identifier.urihttps://doi.org/10.1016/j.saa.2021.119639
dc.identifier.urihttps://hdl.handle.net/20.500.12713/1625
dc.identifier.wosWOS:000639680900001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorBulut, Huri
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofSpectrochim Acta A Mol Biomol Spectrosc .en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAloe Vera Extracten_US
dc.subjectBiochemical Assaysen_US
dc.subjectDiabetic Wound Healingen_US
dc.subjectFTIR Analysisen_US
dc.subjectHypericum Perforatum Oilen_US
dc.subjectInflammationen_US
dc.subjectPolymeric Nanofibers Dressingsen_US
dc.titleSpectrochemical and biochemical assay comparison study of the healing effect of the aloe vera and hypericum perforatum loaded nanofiber dressings on diabetic wounden_US
dc.typeArticleen_US

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