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    Dextran-coated iron oxide nanoparticles in combination with ginger extract without NGF promote neurite outgrowth and PC12 cell branching
    (Academic Press Inc Elsevier Science, 2023) Sadeghi, Sanaz; Esmaeili, Abolghasem; Zarrabi, Ali
    Neurogenesis is decreased in the absence of nerve growth factor (NGF). It would be beneficial to discover substances that stimulate neurogenesis without NGF, given the high molecular weight and brief half-life of NGF. This work aims to assess the neurogenesis of ginger extract (GE) combined with superparamagnetic iron oxide nanoparticles (SPIONs) without NGF. Based on our research, GE and SPIONs start neurogenesis before NGF. In comparison to the control group, GE and SPIONs dramatically reduced the length and quantity of neurites, according to statistical analysis. Our findings also indicated that SPIONs and ginger extract together had an additive impact on one another. The total number significantly increased with the addition of GE and nanoparticles. In comparison to NGF, the mixture of GE and nanoparticles significantly enhanced the total number of cells with neurites (by about 1.2-fold), the number of branching points (by about 1.8-fold), and the length of neurites. The difference between ginger extract and nanoparticles with NGF was significant (about 3.5-fold), particularly in the case of cells with one neurite. The results of this study point to the possibility of treating neurodegenerative disorders via the combination of GE and SPIONs without NGF.
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    Superparamagnetic iron oxide nanoparticles and curcumin equally promote neuronal branching morphogenesis in the absence of nerve growth factor in PC12 cells
    (MDPI, 2022) Zarei, Mahshid; Esmaeili, Abolghasem; Zarrabi, Ali; Zarepour, Atefeh
    Regeneration of the damaged neurons in neurological disorders and returning their activities are two of the main purposes of neuromedicine. Combination use of specific nanoformulations with a therapeutic compound could be a good candidate for neuroregeneration applications. Accordingly, this research aims to utilize the combination of curcumin, as a neurogenesis agent, with dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs) to evaluate their effects on PC12 cells? neuronal branching morphogenesis in the absence of nerve growth factor. Therefore, the effects of each component alone and in combination form on the cytotoxicity, neurogenesis, and neural branching morphogenesis were evaluated using MTT assay, immunofluorescence staining, and inverted microscopy, respectively. Results confirmed the effectiveness of the biocompatible iron oxide nanoparticles (with a size of about 100 nm) in improving the percentage of neural branching (p < 0.01) in PC12 cells. In addition, the combination use of these nanoparticles with curcumin could enhance the effect of curcumin on neurogenesis (p < 0.01). These results suggest that SPIONs in combination with curcumin could act as an inducing factor on PC12 neurogenesis in the absence of nerve growth factor and could offer a novel therapeutic approach to the treatment of neurodegenerative diseases. © 2022 by the authors.