Superparamagnetic iron oxide nanoparticles and curcumin equally promote neuronal branching morphogenesis in the absence of nerve growth factor in PC12 cells
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Regeneration of the damaged neurons in neurological disorders and returning their activities are two of the main purposes of neuromedicine. Combination use of specific nanoformulations with a therapeutic compound could be a good candidate for neuroregeneration applications. Accordingly, this research aims to utilize the combination of curcumin, as a neurogenesis agent, with dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs) to evaluate their effects on PC12 cells? neuronal branching morphogenesis in the absence of nerve growth factor. Therefore, the effects of each component alone and in combination form on the cytotoxicity, neurogenesis, and neural branching morphogenesis were evaluated using MTT assay, immunofluorescence staining, and inverted microscopy, respectively. Results confirmed the effectiveness of the biocompatible iron oxide nanoparticles (with a size of about 100 nm) in improving the percentage of neural branching (p < 0.01) in PC12 cells. In addition, the combination use of these nanoparticles with curcumin could enhance the effect of curcumin on neurogenesis (p < 0.01). These results suggest that SPIONs in combination with curcumin could act as an inducing factor on PC12 neurogenesis in the absence of nerve growth factor and could offer a novel therapeutic approach to the treatment of neurodegenerative diseases. © 2022 by the authors.
Açıklama
Anahtar Kelimeler
Combination Therapy, Curcumin, Neuronal Branching, Neuroregeneration Neuritogenesis, Superparamagnetic Iron Oxide Nanoparticles
Kaynak
Pharmaceutics
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
14
Sayı
12
Künye
Zarei, M., Esmaeili, A., Zarrabi, A., & Zarepour, A. (2022). Superparamagnetic Iron Oxide Nanoparticles and Curcumin Equally Promote Neuronal Branching Morphogenesis in the Absence of Nerve Growth Factor in PC12 Cells. Pharmaceutics, 14(12), 2692.
