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Öğe European Medical Students' Views on Neurosurgery, with Emphasis on South-East Europe (Albania, Greece, Serbia, and Turkey)(Elsevier Inc., 2024) Esen, Aydin, A.; Gazioglu, N.; Tasiou, A.; Mihaylova, S.; Salokorpi, N.; Karampouga, M.; Broekman M.L.D.Background: Neurosurgery, an intricate and dynamic surgical specialty, faces challenges in attracting medical graduates. Despite its potential appeal, a decreasing trend in medical students opting for surgical specialties, including neurosurgery, is noted. This study aims to assess European medical students' perceptions of neurosurgery, focusing on South-East Europe, and address concerns about the declining interest in this field. Methods: A comprehensive digital survey, comprising 33 questions, was distributed to 1115 medical students across 17 European countries. The survey, conducted over 9 months, gathered responses through European neurosurgical societies, the European Association of Neurosurgical Societies (EANS), and university channels. Statistical analysis utilized IBM Statistical Package for the Social Sciences, presenting data through counts, proportions, and ?2 tests. Results: The study reveals that, over the survey period, 834 medical students completed the questionnaire, with a predominant representation from South-East Europe. While 43.2% of participants were considering a surgical career, neurosurgery emerged as the most preferred specialty (26.37%). Despite this interest, 80.2% reported insufficient knowledge about pursuing a neurosurgical career, with limited exposure during medical education. Concerns about work-life balance, heavy workload, and hierarchical structures were prominent among respondents. Conclusions: The findings underscore the need for targeted interventions to address concerns influencing medical students' decisions regarding neurosurgery. Improving neurosurgical education, dispelling misconceptions, and creating a supportive work environment are crucial steps to attract and retain diverse talented individuals in neurosurgery. These efforts will be vital in narrowing the gap between the demand for neurosurgeons and the number of medical graduates entering the field, ensuring a sustainable future for this essential surgical specialty. © 2024 Elsevier Inc.Öğe Occipital Sinus-Sparing Linear Paramedian Dural Incision: A Technical Note and Case Series for Median Suboccipital Approach(Elsevier Inc., 2024) Erol, G.; Çavuşoğlu, N.; Sevgi, U.T.; Fidan, S.; Canbolat, Ç.; Doğruel, Y.; Luzzi S.Background: Durotomies, traditionally used during the midline suboccipital approach, involve sacrificing the occipital sinus (OS) with consequent shrinking of the dura, risk of venous complications, difficulty performing watertight closure, and a higher rate of postoperative cerebrospinal fluid (CSF) leaks. The present technical note describes the OS-sparing linear paramedian dural incision, which leads to a decrease in the risk of complications during the median suboccipital approach in our case series. Methods: The OS-sparing linear incision technique involves a dural incision placed 1 cm lateral to the OS. The angle of view of the microscope is frequently changed to overcome the narrowed exposure of the linear durotomy. Copious irrigation with saline prevents drying of the dura. A running watertight closure of the dura is performed. The overall results of 5 cases are reviewed. Results: The cases were 3 tumors and 2 cavernomas. The OS was preserved in all 5, and no duraplasty was needed. The average dura closure time was 16.8 minutes. No CSF leak occurred, and no wound complications were observed. A gross total resection of the lesion was achieved in all the patients. The mean follow-up was 10.2 months, and there were no late complications related to the dura closure. Conclusions: In comparison to the types of durotomies conventionally used for the midline suboccipital approach, the OS-sparing linear paramedian dural incision entails lower risks of bleeding, venous complications, CSF leaks, and infections by avoiding duraplasty. Validation of this technical note on a larger patient cohort is needed. © 2024 Elsevier Inc.Öğe Cinnamaldehyde has Antifibrotic Effects on Rats with Epidural Fibrosis(Elsevier Inc., 2024) Bektaşoğlu, P.K.; Somay, A.; Hazneci, J.; Borekci, A.; Gürer, B.Background: Laminectomy is a widely employed surgical procedure for the treatment of spinal stenosis, but it may lead to epidural fibrosis (EF) and failed back surgery syndrome. Cinnamaldehyde, a phenylpropanoid found in cinnamon, has demonstrated antioxidant and anti-inflammatory properties. In the present study, we hypothesized that topical application and systemic administration of cinnamaldehyde could be helpful in the prevention of EF in a rat laminectomy model. Methods: The rats were randomly assigned to control, local, and systemic Tween-80 and local and systemic cinnamaldehyde experimental groups (n = 6, per group). In the control group, just laminectomy was performed. In local treatment groups, applications were done just after the laminectomy onto dura. In systemic treatment groups, intraperitoneal administrations were performed following skin suturing. The degree of epidural fibrosis was evaluated macroscopically and histopathologically 4 weeks later. Results: Macroscopic assessment revealed decreased EF with both topical and systemic cinnamaldehyde application, whereas microscopic examination results were not significant. Conclusions: Our findings provide the first experimental evidence of cinnamaldehyde's potential protective effects against EF. © 2023 Elsevier Inc.Öğe Nanomedicine: Patuletin-conjugated with zinc oxide exhibit potent effects against Gram-negative and Gram-positive bacterial pathogens(Springer Science and Business Media B.V., 2024) Khan, N.A.; Alvi, A.; Alqassim, S.; Akbar, N.; Khatoon, B.; Kawish, M.; Faizi S.With the emergence of drug-resistance, there is a need for novel anti-bacterials or to enhance the efficacy of existing drugs. In this study, Patuletin (PA), a flavanoid was loaded onto Gallic acid modified Zinc oxide nanoparticles (PA-GA-ZnO), and evaluated for antibacterial properties against Gram-positive (Bacillus cereus and Streptococcus pneumoniae) and Gram-negative (Samonella enterica and Escherichia coli) bacteria. Characterization of PA, GA-ZnO and PA-GA-ZnO’ nanoparticles was accomplished utilizing fourier-transform infrared spectroscopy, efficiency of drug entrapment, polydispersity index, zeta potential, size, and surface morphology analysis through atomic force microscopy. Using bactericidal assays, the results revealed that ZnO conjugation displayed remarkable effects and enhanced Patuletin’s effects against both Gram-positive and Gram-negative bacteria, with the minimum inhibitory concentration observed at micromolar concentrations. Cytopathogenicity assays exhibited that the drug-nanoconjugates reduced bacterial-mediated human cell death with minimal side effects to human cells. When tested alone, drug-nanoconjugates tested in this study showed limited toxic effects against human cells in vitro. These are promising findings, but future work is needed to understand the molecular mechanisms of effects of drug-nanoconjugates against bacterial pathogens, in addition to in vivo testing to determine their translational value. This study suggests that Patuletin-loaded nano-formulation (PA-GA-ZnO) may be implicated in a multi-target mechanism that affects both Gram-positive and Gram-negative pathogen cell structures, however this needs to be ascertained in future work. © The Author(s), under exclusive licence to Springer Nature B.V. 2024.Öğe Creation of a microsurgical neuroanatomy laboratory and virtual operating room: a preliminary study(American Association of Neurological Surgeons, 2024) Erol, G.; Güngör, A.; Sevgi, U.T.; Gülsuna, B.; Doğruel, Y.; Emmez, H.; Türe U.OBJECTIVE A comprehensive understanding of microsurgical neuroanatomy, familiarity with the operating room environment, patient positioning in relation to the surgery, and knowledge of surgical approaches is crucial in neurosurgical education. However, challenges such as limited patient exposure, heightened patient safety concerns, a decreased availability of surgical cases during training, and difficulties in accessing cadavers and laboratories have adversely impacted this education. Three-dimensional (3D) models and augmented reality (AR) applications can be utilized to depict the cortical and white matter anatomy of the brain, create virtual models of patient surgical positions, and simulate the operating room and neuroanatomy laboratory environment. Herein, the authors, who used a single application, aimed to demonstrate the creation of 3D models of anatomical cadaver dissections, surgical approaches, patient surgical positions, and operating room and laboratory designs as alternative educational materials for neurosurgical training. METHODS A 3D modeling application (Scaniverse) was employed to generate 3D models of cadaveric brain specimens and surgical approaches using photogrammetry. It was also used to create virtual representations of the operating room and laboratory environment, as well as the surgical positions of patients, by utilizing light detection and ranging (LiDAR) sensor technology for accurate spatial mapping. These virtual models were then presented in AR for educational purposes. RESULTS Virtual representations in three dimensions were created to depict cadaver specimens, surgical approaches, patient surgical positions, and the operating room and laboratory environment. These models offer the flexibility of rotation and movement in various planes for improved visualization and understanding. The operating room and laboratory environment were rendered in three dimensions to create a simulation that could be navigated using AR and mixed reality technology. Realistic cadaveric models with intricate details were showcased on internet-based platforms and AR platforms for enhanced visualization and learning. CONCLUSIONS The utilization of this cost-effective, straightforward, and readily available approach to generate 3D models has the potential to enhance neuroanatomical and neurosurgical education. These digital models can be easily stored and shared via the internet, making them accessible to neurosurgeons worldwide for educational purposes. © AANS 2024, except where prohibited by US. All Rights Reserved.Öğe Structure-based drug design of DNA minor groove binders and evaluation of their antibacterial and anticancer properties(Elsevier Masson s.r.l., 2024) Alniss, H.Y.; Al-Jubeh, H.M.; Msallam, Y.A.; Siddiqui, R.; Makhlouf, Z.; Ravi, A.; Hamdy R.Antimicrobial and chemotherapy resistance are escalating medical problem of paramount importance. Yet, research for novel antimicrobial and anticancer agents remains lagging behind. With their reported medical applications, DNA minor groove binders (MGBs) are worthy of exploration. In this study, the approach of structure-based drug design was implemented to generate 11 MGB compounds including a novel class of bioactive alkyne-linked MGBs. The NCI screening protocol was utilized to evaluate the antitumor activity of the target MGBs. Furthermore, a variety of bactericidal, cytopathogenicity, MIC90, and cytotoxicity assays were carried out using these MGBs against 6 medically relevant bacteria: Salmonella enterica, Escherichia coli, Serratia marcescens, Bacillus cereus, Streptococcus pneumoniae and Streptococcus pyogenes. Moreover, molecular docking, molecular dynamic simulations, DNA melting, and isothermal titration calorimetry (ITC) analyses were utilized to explore the binding mode and interactions between the most potent MGBs and the DNA duplex d(CGACTAGTCG)2. NCI results showed that alkyne-linked MGBs (26 & 28) displayed the most significant growth inhibition among the NCI-60 panel. In addition, compounds MGB3, MGB4, MGB28, and MGB32 showed significant bactericidal effects, inhibited B. cereus and S. enterica-mediated cytopathogenicity, and exhibited low cytotoxicity. MGB28 and MGB32 demonstrated significant inhibition of S. pyogenes, whereas MGB28 notably inhibited S. marcescens and all four minor groove binders significantly inhibited B. cereus. The ability of these compounds to bind with DNA and distort its groove dimensions provides the molecular basis for the allosteric perturbation of proteins-DNA interactions by MGBs. This study shed light on the mechanism of action of MGBs and revealed the important structural features for their antitumor and antibacterial activities, which are important to guide future development of MGB derivatives as novel antibacterial and anticancer agents. © 2024 Elsevier Masson SASÖğe Antiamoebic properties of ceftriaxone and zinc-oxide-cyclodextrin-conjugated ceftriaxone(MDPI, 2022) Makhlouf, Zinb; Akbar, Noor; Khan, Naveed Ahmed; Shah, Muhammad Raza; Alharbi, Ahmad M.; Alfahemi, Hasan; Siddiqui, RuqaiyyahAcanthamoeba castellanii is a ubiquitous free-living amoeba capable of instigating keratitis and granulomatous amoebic encephalitis in humans. Treatment remains limited and inconsistent. Accordingly, there is a pressing need for novel compounds. Nanotechnology has been gaining attention for enhancing drug delivery and reducing toxicity. Previous work has shown that various antibiotic classes displayed antiamoebic activity. Herein, we employed two antibiotics: ampicillin and ceftriaxone, conjugated with the nanocarrier zinc oxide and beta-cyclodextrin, and tested them against A. castellanii via amoebicidal, amoebistatic, encystment, excystment, cytopathogenicity, and cytotoxicity assays at a concentration of 100 mu g/mL. Notably, zinc oxide beta-cyclodextrin ceftriaxone significantly inhibited A. castellanii growth and cytopathogenicity. Additionally, both zinc oxide beta-cyclodextrin ceftriaxone and ceftriaxone markedly inhibited A. castellanii encystment. Furthermore, all the tested compounds displayed negligible cytotoxicity. However, minimal anti-excystment or amoebicidal effects were observed for the compounds. Accordingly, this novel nanoconjugation should be employed in further studies in hope of discovering novel anti-Acanthamoeba compounds.Öğe Treatment of isolated zygomatic arch fractures with the gillies method(Springer, 2023) Çil, YakupGillies method is widely used for zygomatic arch fractures. Many modifications were suggested over time but Gillies method is still carry on popularity. Seventeen patients with isolated zygomatic arch fractures who were treated with Gillies method between 2016 and 2021 were included in this study. All patients were male and the mean age was 23 years (20-32). All operations were performed under sedation anesthesia. All patients healed uneventfully. Only one transient frontal branch of facial nerve paralysis was seen in the follow-up. Etiology of zygomatic arc fractures were sports injury in 9 patients (53%), motorcycle accident in 3 patients (17.6%), assault in 3 patients (17.6), and sencop in 2 patients (11.7%). Zygomatic arch fractures were located on left zygomatic arch in 13 patients (76.5%), right zygomatic arch in 4 patients (23.5%). Eleven patients (64.7%) were operated in 1 week and 6 patients were operated on the second week (35.3%) after the injury. We think that Gillies method is still very useful in the treatment of isolated zygomatic arch fractures.Öğe Postoperative pulmonary complications after percutaneous nephrolithotomy under spinal anesthesia(2022) Kara, Burak; Önem, Kadir; Çetinkaya, Mehmet; Açıkgöz, AbdullahPurpose: To evaluate risk factors and outcomes of Pulmonary Complications (PCs) in Percutaneous Nephrolithotomy (PCNL) under Spinal anesthesia (SA). Material and method: 286 patients who underwent PCNL under SA between 2017 and 2021 were identified retrospectively and divided into group 1 (clinically significant PCs) and group 2 (no clinically significant PCs). Demographic, preoperative, and intraoperative variables and postoperative outcomes were compared between both groups. Independent risk factors for PCs were evaluated by univariable and multivariable logistic regression analyses. Results: PCs were noted in 90 patients (31.5%). Advanced age (P = .011), high body mass index (BMI) (P < .001), and the presence of chronic obstructive pulmonary disease (COPD) (P < .001) were risk factors for PCs. Conclusion: SA is an effective method of anesthesia for all PCNL patients and carries a lower rate of PCNL-associated PCs. Risk factors for PCs after PCNL were advanced age, obesity, and preoperative COPD.Öğe Effectiveness of ozone-oxygen mixture by ultrasonography-guided facet medial branch block in the treatment of cervicogenic dizziness(Wolters Kluwer Medknow Publications, 2022) Yılmaz, Mahmut Sinan; Demir, Gürkan; Kocayi?it, Havva; E?ilmez, O?uz Kadir; Beyaz, Serbülent GökhanPurpose: Among many central and peripheral causes of vertigo, one of the factors whose pathophysiology is not yet understood is cervicogenic dizziness (CD). There are studies reporting that CD may benefit from facet joint medial bundle branch block (MBB). The purpose of our study was to share our experiences of MBB application using ultrasonography (USG) in patients diagnosed with CD. Materials and Methods: After physical examination and necessary cervical imaging, C1, C2 and C3 cervical facet joint MBB and ozone injection were performed using USG for patients who had vertigo and accompanying cervical pathology and neck pain. In order to evaluate the effectiveness of the treatment, pain levels with the Visual Analog Scale (VAS) and the effect of vertigo on the quality of life with the 'Dizziness Handicap Inventory (DHI)' Turkish validation questionnaire were documented. Results: Thirty-five patients who met the criteria were included in the study. The mean age of the patients was 50.8 ± 10.7. Of the 35 patients, 10 (28.6%) were male and 25 (71.4%) were female. There was a significant difference between the preoperative period and the postoperative 1 st, 2 nd, and 6 th week VAS scores. A significant difference was found between the preoperative and the postoperative 2 nd and 6 th week DHI questionnaire survey results as well. Conclusions: In the follow-up after the procedures, it was observed that vertigo complaints of the patients decreased significantly. © 2022 Indian Journal of Otology | Published by Wolters Kluwer - Medknow.Öğe Effect of microgravity on the gut microbiota bacterial composition in a hindlimb unloading model(MDPI, 2022) Siddiqui, Ruqaiyyah; Qaisar, Rizwan; Khan, Naveed Ahmed; Alharbi, Ahmad M.; Alfahemi, Hasan; Elmoselhi, AdelWe utilised a ground-based microgravity hindlimb unloading (HU) mouse model to elucidate the gut microbiota bacterial changes in mice under a simulated microgravity environment. Four-month-old, male C57/Bl6 mice were randomly divided into ground-based controls and the HU groups and kept under controlled environmental conditions. For the microgravity environment, the mice were suspended in special cages individually for 20 days. At the end of the suspension, the mice were sacrificed; gut dissections were performed, followed by a metagenomic analysis of bacterial species, which was carried out by extracting DNA and 16S rRNA analysis. The results revealed that the gut bacterial communities of mice under gravity and microgravity were different. Notably, our findings revealed differences in the bacterial community structure. Around 449 bacterial OTUs were specific to mice kept under normal gravity versus 443 bacterial OTUs under microgravity conditions. In contrast, 694 bacterial OTUs were common to both groups. When the relative abundance of taxa was analyzed, Bacteroidetes dominated the gut (64.7%) of normal mice. Conversely, mice in the microgravity environment were dominated by Firmicutes (42.7%), and the relative abundance of Bacteroidetes differed significantly between the two groups (p < 0.05). The distribution of Muribaculaceae between normal mice versus microgravity mice was significantly different, at 62% and 36.4%, respectively (p < 0.05). Furthermore, a significant decrease in 11 bacteria was observed in mice under simulated microgravity, including Akkermansia muciniphila, Eubacterium coprostanoligenes, Bacteroides acidifaciens, Clostridium leptum, Methylorubrum extorquens, Comamonas testosterone, Desulfovibrio fairfieldensis, Bacteroides coprocola, Aerococcus urinaeequi, Helicobacter hepaticus, and Burkholderiales. Further studies are needed to elucidate gut bacterial metabolites of these identified bacterial species in microgravity conditions and normal environment. Notably, the influence of these metabolites on obesity, neuroprotection, musculoskeletal and cardiovascular dysfunction, longevity, inflammation, health, and disease in astronauts ought to be investigated and will be important in developing procedures against adverse effects in astronauts following space travel.Öğe Recombinant FSH versus highly purified urinary FSH in patients with polycystic ovary syndrome undergoing ICSI cycles: a prospective randomized study(Galenos, 2022) Aynıoğlu, Öner; Ceylan, Yasin; Akar, Bertan; Özkan, Sabiha; Çalışkan, ErayObjective: To compare efficacy and safety of recombinant follicule stimulating hormone (r-FSH) and highly purified urinary FSH (HP-uFSH) in polycystic ovary syndrome (PCOS) patients undergoing intracytoplasmic sperm injection (ICSI). Method: This was a prospective randomized study conducted at Kocaeli University Faculty of Medicine, Department of Obstetrics and Gynecology, in vitro fertizilization (IVF) Unit. A total of 91 PCOS patients undergoing ICSI were randomly assigned to receive either r-FSH (n = 46) or HPuFSH (n = 45) with a gonadotropin releasing hormone (GnRH) antagonist protocol. The main outcome measures were the number of mature oocytes retrieved, embryo quality, pregnancy rates, implantation rates. Results: The number of mature oocytes retrieved, fertilization rates, the number of cryopreserved embryos were significantly higher in r-FSH group (p = 0.024, p = 0.023, p = 0.026 respectively) while the total dose of FSH used was significantly lower in the same group (p = 0.023). Pregnancy rates, clinical pregnancy rates were higher in r-FSH group although not statistically significant (52.2% versus 35.6%, p = 0.11, 37% versus 28.9%, p = 0.41 respectively). Overall therapy costs per clinical pregnancy were associated with a 9.94% increase in r-FSH group whereas costs per pregnancy were not different between groups. Conclusion: r-FSH is superior than HP-uFSH in PCOS regarding fertilization rates, the number of mature oocytes retrieved and cryopreserved embryos, pregnancy rates although overall therapy costs per clinical pregnancy are higher. Keywords: ART, HP-uFSH, PCOS, rec-FSH Amac: ICSI uygulanan PCOS hastalarinda rekombinant FSH (r-FSH) ve yuksek oranda saflastirilmis uriner FSH'nin (HP-uFSH) etkinligini ve guvenligini karsilastirmaktir. Yontem: Kocaeli Universitesi Tip Fakultesi Kadin Hastaliklari ve Dogum Anabilim Dali Tup Bebek Unitesi'nde yurutulen prospektif randomize bir calismadir. ICSI uygulanan toplam 91 PCOS hastasi, bir GnRH antagonist protokolu ile r-FSH (n = 46) ve HP-uFSH (n = 45) almak uzere rastgele belirlendi. Ana sonuc olcutleri; alinan olgun oosit sayisi, embriyo kalitesi, gebelik oranlari, implantasyon oranlariydi. Bulgular: Alinan olgun oosit sayisi, dollenme oranlari, dondurularak saklanan embriyo sayisi r-FSH grubunda anlamli olarak daha yuksek (sirasiyla p = 0,024, p = 0,023, p = 0,026), ayni grupta kullanilan toplam FSH dozu ise anlamli olarak daha dusuktu (p = 0,023). Gebelik oranlari, klinik gebelik oranlari r-FSH grubunda istatistiksel olarak anlamli olmamakla birlikte daha yuksekti (sirasiyla %52,2'ye karsi %35,6, p = 0,11, %37'ye karsi %28,9, p = 0,41). Klinik gebelik basina genel tedavi maliyetleri, r-FSH grubunda %9,94'luk bir artisla iliskilendirilirken, gebelik basina maliyetler gruplar arasinda farkli degildi. Sonuc: r-FSH, PCOS'de fertilizasyon oranlari, alinan olgun oosit sayisi ve dondurularak saklanan embriyolar, gebelik oranlari acisindan HP-uFSH'den ustundur, ancak klinik gebelik basina genel tedavi maliyetleri daha yuksektir. Anahtar kelimeler: ART, HP-uFSH, PCOS, rec-FSHÖğe Humoral and cellular immune response to SARS-CoV-2 mRNA BNT162b2 vaccine in pediatric kidney transplant recipients compared with dialysis patients and healthy children(SPRINGER, 2022) Gülmez, Rüveyda; Özbey, Doğukan; Ağbaş, Ayşe; Aksu, Bağdagül; Yıldız, Nurdan; Üçkardeş, Diana; Taşdemir, MehmetBackground: Compared with the general population, the immune response to COVID-19 mRNA vaccines is lower in adult kidney transplant recipients (KTRs). However, data is limited for pediatric KTRs. In this study, we aimed to assess humoral and cellular immune responses to the COVID-19 mRNA vaccine in pediatric KTRs. Methods: This multicenter, prospective, case-control study included 63 KTRs (37 male, aged 12-21 years), 19 dialysis patients, and 19 controls. Humoral (anti-SARS-CoV2 IgG, neutralizing Ab (nAb)) and cellular (interferon-gamma release assay (IGRA)) immune responses were assessed at least one month after two doses of BNT162b2 mRNA vaccine. Results: Among COVID-19 naïve KTRs (n = 46), 76.1% tested positive for anti-SARS-CoV-2 IgG, 54.3% for nAb, and 63% for IGRA. Serum levels of anti-SARS-CoV-2 IgG and nAb activity were significantly lower in KTRs compared to dialysis and control groups (p < 0.05 for all). Seropositivity in KTRs was independently associated with shorter transplant duration (p = 0.005), and higher eGFR (p = 0.007). IGRA titer was significantly lower than dialysis patients (p = 0.009). Twenty (43.4%) KTRs were positive for all immune parameters. Only four of 11 seronegative KTRs were IGRA-positive. COVID-19 recovered KTRs had significantly higher anti-SARS-CoV-2 IgG and nAb activity levels than COVID-19 naïve KTRs (p = 0.018 and p = 0.007, respectively). Conclusions: The humoral and cellular immune responses to SARS-CoV-2 mRNA BNT162b2 vaccine are lower in pediatric KTRs compared to dialysis patients. Further prospective studies are required to demonstrate the clinical efficacy of the mRNA vaccine in KTRs. This prospective study was registered in ClinicalTrials.gov (NCT05465863, registered retrospectively at 20.07.2022). A higher resolution version of the Graphical abstract is available as Supplementary information.Öğe Cockroaches: a potential source of novel bioactive molecule(s) for the benefit of human health(SPRINGER, 2022) Siddiqui, Ruqaiyyah; Yara Elmashak; Khan, Naveed AhmedCockroaches are one of the hardiest insects that have survived on this planet for millions of years. They thrive in unhygienic environments, are able to survive without food for up to 30 days, without air for around 45 min and being submerged under water for 30 min. Cockroaches are omnivorous and feed on a variety of foods, including cellulose and plastic, to name a few. It is intriguing that cockroaches are able to endure and flourish under conditions that are harmful to Homo sapiens. Given the importance of the gut microbiome on its' host physiology, we postulate that the cockroach gut microbiome and/or its metabolites, may be contributing to their "hardiness", which should be utilized for the discovery of biologically active molecules for the benefit of human health. Herein, we discuss the biology, diet/habitat of cockroaches, composition of gut microbiome, cellular senescence, and resistance to infectious diseases and cancer. Furthermore, current knowledge of the genome and epigenome of these remarkable species is considered. Being one of the most successful and diverse insects, as well as their extensive use in traditional and Chinese medicine, the lysates/extracts and gut microbial metabolites of cockroaches may offer a worthy resource for novel bioactive molecule(s) of therapeutic potential for the benefit of human health and may be potentially used as probiotics.Öğe Interleukin-21 and interleukin-32 gene expression levels and their relationship with clinicopathological parameters in colorectal cancer(EDIZIONI LUIGI POZZI, 2022) Özmen, Füsun; Erdem, Gizem Övgü; Kulaçoğlu, Sezer; Özmen, Mehmet Mahir; Kansu, EminBACKGROUND AND AIMS: The immune cells in tumor microenvironment release chemokines and cytokines which determine the immune phenotype of the tumor and play an important role in the prognosis. Present study evaluates the gene expression levels of IL -21 and IL -32 and their relations to clinicopathologic parameters in colorectal cancer. PATIENTS AND METHODS: 31(17F) patients with colorectal cancer were included. Samples were obtained from normal and tumor tissues. After RNA isolation, IL -21 and IL -32 gene expression levels were measured. Immunohistochemistry was also carried out for CD4+, CD8+ and NKcells to measure cell density. The relations between expression levels, immune cell density and diffrentiation, stage, presence of vascular, perineural invasion and lymph node metastasis(MLN) were investigated. RESULTS: IL -32 gene expression levels were increased in tumor tissues. IL -21 levels were found to be decreased in 50% of the patients. IL -32 levels were also increased with the stage however, it was decreased significantly with the increased number of the MLIV. On the other hand, expression levels of IL -21 increased significantly with the presence of vascular invasion. CD4+ density was decreased with increased T-stage, vascular invasion whereas CD8+ density decreased only with the vascular invasion. CONCLUSIONS: IL -32 expressed by tumor microenvironment reveals that expression increased to control tumor growth, but levels are decreased with the increased number of MLNs which might be due to decreased CD4+ cell density. Changes on IL -21 and IL -32 together with the changes on immune cell density, indicate their role in tumor growth and invasion in colon cancer.Öğe Acousto-holographic reconstruction of whole-cell stiffness maps(Nature Research, 2022) Varol, Rahmetullah; Karavelioğlu, Zeynep; Ömeroğlu, Sevde; Aydemir, Gizem; Karadağ, Aslıhan; Meco, Hanife E.; Demirçalı, Ali A.; Yılmaz, Abdurrahim; Koçal, Gizem C.; Gençoğlan, Gülsüm; Oruç, Muhammed E.; Esmer, Gökhan B.; Başbınar, Yasemin; Özdemir, Şahin K.; Üvet, HüseyinAccurate assessment of cell stiffness distribution is essential due to the critical role of cell mechanobiology in regulation of vital cellular processes like proliferation, adhesion, migration, and motility. Stiffness provides critical information in understanding onset and progress of various diseases, including metastasis and differentiation of cancer. Atomic force microscopy and optical trapping set the gold standard in stiffness measurements. However, their widespread use has been hampered with long processing times, unreliable contact point determination, physical damage to cells, and unsuitability for multiple cell analysis. Here, we demonstrate a simple, fast, label-free, and high-resolution technique using acoustic stimulation and holographic imaging to reconstruct stiffness maps of single cells. We used this acousto-holographic method to determine stiffness maps of HCT116 and CTC-mimicking HCT116 cells and differentiate between them. Our system would enable widespread use of whole-cell stiffness measurements in clinical and research settings for cancer studies, disease modeling, drug testing, and diagnostics. © 2022, The Author(s).Öğe Application of the free-style brachial artery perforator based propellar flap in the management of axillary hidradenitis suppurativa(WILEY, 2022) Datlı, Aslı; Demirel, OğuzhanHidradenitis suppurativa (HS) is a chronic and recurrent inflammatory disease often requiring surgical intervention in extensive lesions. Total of seven axillary lesion with HS was treated with free-style perforator based propeller flaps (innominate perforator originating from the brachial artery) after complete excision. The flap design mimicked the axis and design of the brachioplasty surgery. The flaps were elevated and after obliterating the defect, the remnant flap was discarded and donor site was primarily closed. All flaps were performed successfully and HS did not recur in any of the patients. In addition, the operated limb also showed a reduction of arm circumference of more than 5 cm. This is the first report using the free-style perforator based propeller flap to reconstruct the axillary HS. It is a simple and a reliable approach yielding good functional and aesthetic outcome with additional advantages of providing a brachioplasty.Öğe Gender differences in work-life balance of European neurosurgeons(Elsevier Ltd., 2022) Lambrianou, Xanthoula; Tzerefos,Christos; Janssen, Insa K; Mihaylova, Stiliana; Aydın,Ayşegül Esen; Al-Ahmad, Selma; Broekman, Marike Ld; Gazioğlu, Nurperi; Duran, Silvia Hernandez; Ivan, Daniela Luminita; Karampouga, Maria; Magnadottir, Hulda B; Pajaj ,Ermira; Rodríguez-Hernández, Ana; Rosseau, Gail; Salokorpi, Niina; Tsianaka, Eleni; Vayssiere, Pia; Murphy, Mary; Tasiou, AnastasiaGender differences in work-life balance of European neurosurgeonsÖğe Adli tıbbi sürece yansıyan femur-humerus kırığı saptanan yenidoğanların retrospektif değerlendirilmesi: tanımlayıcı çalışma(Turkiye Klinikleri J Foren Sci Leg Med., 2022) Yıldız Silahlı,Nicel; Aslıyüksek, Hızır; Dikici,Fatih; Celkan, Tülin TirajeAmaç: Doğum travmaları, doğum eylemi esnasında mekanik etkiler ile ortaya çıkan fiziksel yaralanmalardır. Doğum sırasında karşılaşılan bu durum medikolegal sorunlara neden olmaktadır. Çalışmamızda, yenidoğan döneminde uzun kemik kırığı saptanarak medikolegal sürece konu olmuş olguların özelliklerinin tartışılması amaçlanmıştır. Gereç ve Yöntemler: Adli Tıp Kurumu 7. İhtisas Kurulunda 2018-2020 yılları arasında medikolegal değerlendirme yapılan uzun kemik (femur/humerus) kırığı geliştiği iddiası bulunan olgular (n=23) dâhil edildi. Olguların sosyodemografik ve klinik özellikleri ile kurul kararları retrospektif olarak incelendi. Çalışma izni 01 Aralık 2020 tarih ve 1114 sayılı Adli Tıp Kurumu Bilimsel Araştırma Komisyonundan alındı. Bulgular: Olguların kız/erkek oranı 8/15 idi. On sekiz (%78,2) raporda hekim şikâyeti vardı. Olgularda femur (n=14) ve humerus (n=9) kırıkları saptandı. Olguların gestasyon haftası ortalaması 33 idi. Olguların ortalama doğum tartıları 2.921 (750-5.140 g) g saptandı. Olguların, term/preterm oranı 18/5 saptandı. Humerus ve femur kırığı saptanan 23 olguda da konservatif tedavi uygulanmıştı. Yirmi iki olguda tıbbi uygulama hatası saptanmadı ve kırıklar komplikasyon olarak değerlendirildi. Sonuç: Medikolegal sürece yansıyan uzun kemik kırıklarının tanı ve takip sürecinin uygun şekilde yönetildiği görülmektedir. Süreçte hasta-hekim arasında yaşanan iletişim sorunlarının güven ilişkisini sarstığı görülmektedir. Tıbbi bakım ve tedavi sırasında meydana gelen ve direkt olarak hekimin hatalı bir eylemi sonucu oluşmayan bu durum doğum süreci açısından komplikasyon olarak tanımlanmaktadır.Öğe Imidazothiazole derivatives exhibited potent effects against brain-eating amoebae(MDPI, 2022) Siddiqui, Ruqaiyyah; El-Gamal, Mohammed, I; Boghossian, Anania; Saeed, Balsam Qubais; Oh, Chang-Hyun; Abdel-Maksoud, Mohammed S.; Alharbi, Ahmad M.; Alfahemi, Hasan; Khan, Naveed AhmedNaegleria fowleri (N. fowleri) is a free-living, unicellular, opportunistic protist responsible for the fatal central nervous system infection, primary amoebic meningoencephalitis (PAM). Given the increase in temperatures due to global warming and climate change, it is estimated that the cases of PAM are on the rise. However, there is a current lack of awareness and effective drugs, meaning there is an urgent need to develop new therapeutic drugs. In this study, the target compounds were synthesized and tested for their anti-amoebic properties against N. fowleri. Most compounds exhibited significant amoebicidal effects against N. fowleri; for example, 1h, 1j, and 1q reduced N. fowleri's viability to 15.14%, 17.45% and 28.78%, respectively. Furthermore, the majority of the compounds showed reductions in amoeba-mediated host death. Of interest are the compounds 1f, 1k, and 1v, as they were capable of reducing the amoeba-mediated host cell death to 52.3%, 51%, and 56.9% from 100%, respectively. Additionally, these compounds exhibit amoebicidal properties as well; they were found to decrease N. fowleri's viability to 26.41%, 27.39%, and 24.13% from 100%, respectively. Moreover, the MIC50 values for 1e, 1f, and 1h were determined to be 48.45 mu M, 60.87 mu M, and 50.96 mu M, respectively. Additionally, the majority of compounds were found to exhibit limited cytotoxicity, except for 1l, 1o, 1p, 1m, 1c, 1b, 1zb, 1z, 1y, and 1x, which exhibited negligible toxicity. It is anticipated that these compounds may be developed further as effective treatments against these devastating infections due to brain-eating amoebae.
