Moleküler Biyoloji ve Genetik Bölümü Bildiri Koleksiyonu
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Öğe Identification of microenvironmental regulation and therapeutic targeting of ongenic EF-2 kinase in pancreatic cancer(Science Direct, 2018) Karakaş Zeybek, Didem; Kahraman, Nermin; Bayraktar, Recep; Kabil, Nashwa; Ulukaya, Engin; Dere, Egemen; Lopez-Berestein, Gabriel; Özpolat, BülentIntroduction Pancreatic cancer (PaCa) is one of the most aggressive and deadliest cancer with 6 months average survival rate. Recent studies indicate that the tumour microenvironment (TME) plays an important role in all stages of tumorigenesis in PaCa. Therefore, both tumour cells and their TME should be targeted for an effective treatment. Eukaryotic elongation factor 2 kinase (EF2K) is an enzyme which is overexpressed in cancer cells and plays a key role in cancer cell survival under stress conditions. There is no study that have shown the relationship between EF2K and TME. Thus, we investigated that the effects of EF2K expression on PaCa cell line (PANC1) and the TME.Öğe Encapsulation of in vitro-derived propagules of two genotypes of Capparis spinosa (L.) from Pantelleria Island(International Society for Horticultural Science, 2020) Germana, Maria Antonietta; Lesto, F.; Lo Bosco, Fabrizia; Iacuzzi, Nicolo; Özüdoğru, Elif AylinEncapsulation technology, combining the advantages of zygotic or gamic seed with those of micropropagation, has recently attracted the interest of researchers as a new propagation approach, mainly due to the unsatisfying results of the traditional propagation strategies. The encapsulation of uninodal microcuttings (3-4 mm long) from two Sicilian Capparis spinosa (L.) genotypes (from Pantelleria Island: 'Tracino' and 'Scauri') was evaluated, observing the influence of the calcium alginate coating and of three different growth regulators (PGRs): 6-benzylaminopurine (BAP), meta-topolin (MT) and zeatin (ZEA), on viability, regrowth and conversion of the propagules. Caper microcuttings were dissected and placed in different Murashige and Skoog-based artificial endosperms, enriched of either MT, ZEA or BAP. The synthetic seeds obtained were sown on a medium with full strength salts concentration, enriched with 0.4 mg L-1of naphtalene acetic acid and 0.7 mg L-1of gibbelleric acid. After 60 days, the following parameters were detected: viability, regrowth, number and length of the shoots and roots, conversion. The results confirm that encapsulation did not negatively affect the viability, which showed the highest percentage with BAP (100%) in 'Tracino' and with ZEA (100%) in 'Scauri'. Similar results were obtained in regrowth, with statistically significant differences among the three PGRs tested: 'Tracino' showed the best regrowth on capsuled enriched with BAP (100%), 'Scauri' with ZEA (100%). In addition, the synseed conversion was greatly affected by the PGR, and it was higher in artificial endosperm added with BAP in 'Tracino' (56.6%) and ZEA in 'Scauri' (23.3%) genotype.Öğe Dual inhibition of Wnt/beta-catenin signaling and histone deacetylation as a new strategy to eliminate breast cancer stem cells by augmentation of apoptosis(Wiley, 2018) Aztopal, Nazlıhan; Erkısa Genel, Merve; Arı, Ferda; Dere, E.; Ulukaya, Engin[No Abstract Available]Öğe New gene expression regulators: long non-coding RNAs(Amer Inst Physics, 2019) Gözükırmızı, Nermin; Karlık, ElifLong non-coding RNAs (lncRNAs) play key roles in the regulation of biological processes including response to environmental stresses either transcriptional or post-transcriptional levels. The salinity tolerance of barley may play an important role in understanding the salinity mechanism. The aim of this study is the investigation of molecular responses of four barley genotypes Hordeum vulgare L. (Hasat, Beysehir 99, Konevi 98 and Tarm 92) to 150 mM salinity during 3 days of germination period. To reveal the effect of lncRNAs, we performed Real-time PCR and fluorescence in situ hybridization (FISH) studies. Study demonstrated salinity affected the expression levels of four lncRNAs, CNT0018772, CNT0031477, AK363461, and AK370506, during germination. Previous studies have shown that there was no statistical difference between barley varieties for expression level of CNT0018772 (p>0.05). However, our study indicated that there was statistically significant difference between barley varieties for CNT0031477 (p<0.05). Additionally, there was statistically significant difference between barley varieties for the two barley lncRNAs of AK363461 and AK370506, (p<0.05). We were also able to observe the localization of these four lncRNAs on barley chromosomes, which were exhibited under confocal microscope via in situ hybridization on barley root preparations. This study further supports the reasons why nowadays, lncRNAs are considered as major regulators.Öğe Identification of microenvironmental regulation and therapeutic targeting of oncogenic eEF2K in pancreatic cancer(Wiley, 2019) Karakaş Zeybek, Didem; Kahraman, Nermin; Bayraktar, Recep; Kabil, Nashwa; Ulukaya, Engin; Dere, Egemen; Berestein, Gabriel Lopez; Özpolat, BülentEukaryotic elongation factor 2 kinase (eEF2K) is overexpressed in cancer cells and its overexpression is correlated with poor prognosis in several types of cancer. It has been known that pancreatic cancer (PaCa) has a unique tumor microenvironment (TME) which contributes all stages of tumorigenesis. In this study, the effects of interaction between PaCa cells and macrophages on eEF2K levels and aggressive tumor behaviours were investigated.
