INFECTIVITY AND REPLICATION INHIBITION EFFECT OF 5-FLUOROURACIL ON HERPES SIMPLEX VIRUS TYPE-1 ASSOCIATED WITH MUTATIONS IN THYMIDINE KINASE GENE

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dc.authoridTAMER, Cüneyt/0000-0003-3240-8425
dc.authoridÖZAN, Emre/0000-0001-9872-8152
dc.authoridYAZICI, Zafer/0000-0002-2806-7878
dc.authoridElhag, Ahmed Eisa/0000-0002-0738-1825
dc.authoridALBAYRAK, Harun/0000-0002-4468-2790
dc.authorwosidTAMER, Cüneyt/AFH-9368-2022
dc.authorwosidÖZAN, Emre/AAB-8896-2020
dc.authorwosidYAZICI, Zafer/AAV-5880-2021
dc.authorwosidElhag, Ahmed Eisa/AAU-7483-2020
dc.contributor.authorTaskin, Mehmet Hakan
dc.contributor.authorKurucay, Hanne Nur
dc.contributor.authorKadi, Hamza
dc.contributor.authorTamer, Cuneyt
dc.contributor.authorOzan, Emre
dc.contributor.authorMuftuoglu, Bahadir
dc.contributor.authorAlbayrak, Harun
dc.date.accessioned2024-05-20T14:38:45Z
dc.date.available2024-05-20T14:38:45Z
dc.date.issued2022
dc.departmentİstinye Üniversitesien_US
dc.description.abstractTwo pandemics over the first two decades of the 21st century have shown that viruses with epidemic and pandemic potentials constitute a major threat to human health due to the lack of effective antivirals. Developing new antivirals and treatment strategies is also as important as vaccines play a crucial role in the first line of preventing viral infections. Using base analogues that directly target viruses and understanding their action mechanisms can present essential alternative treatment strategies to combat viral diseases. The current study investigated the effects of a base analogue on herpes simplex virus type1 (HSV-1) replication in a cell culture system using the pyrimidine analogue 5-fluorouracil (FU). After that, the full-length UL-23 gene encoding thymidine kinase (TK) of HSV-1 was sequenced to detect induced mutations. The results showed a diminishing viral titre and viral load at 2 logs and 663 times, respectively, at the end of 10 consecutive passages with 5-FU. Furthermore, two mutations substitute amino acids in the non-conserved region of the TK gene, which confers drug resistance, were also identified. The current research is a feasibility study to investigate the antiviral effects of 5-FU on DNA viruses and has reinforced the fact that 5-FU can have an antiviral effect on HSV-1. However, drug resistance for viruses should not be underestimated.en_US
dc.identifier.doi10.31925/farmacia.2022.6.14
dc.identifier.endpage1113en_US
dc.identifier.issn0014-8237
dc.identifier.issn2065-0019
dc.identifier.issue6en_US
dc.identifier.startpage1105en_US
dc.identifier.urihttps://doi.org10.31925/farmacia.2022.6.14
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4591
dc.identifier.volume70en_US
dc.identifier.wosWOS:000969664100014en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSoc Stiinte Farmaceutice Romaniaen_US
dc.relation.ispartofFarmaciaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subject5-Fluorouracilen_US
dc.subjectAntiviralen_US
dc.subjectHerpes Simplex Virusen_US
dc.subjectThymidine Kinaseen_US
dc.subjectUl-23en_US
dc.titleINFECTIVITY AND REPLICATION INHIBITION EFFECT OF 5-FLUOROURACIL ON HERPES SIMPLEX VIRUS TYPE-1 ASSOCIATED WITH MUTATIONS IN THYMIDINE KINASE GENEen_US
dc.typeArticleen_US

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