Drug modifications: graphene oxide-chitosan loading enhanced anti-amoebic effects of pentamidine and doxycycline

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dc.authorscopusidNaveed Ahmed Khan / 7401797675
dc.authorscopusidRuqaiyyah Siddiqui / 18635417000
dc.authorwosidNaveed Ahmed Khan / FXL-5617-2022
dc.authorwosidRuqaiyyah Siddiqui / AIF-2100-2022
dc.contributor.authorJabri, Tooba
dc.contributor.authorDaalah, Meshal
dc.contributor.authorAlawfi, Bader S.
dc.contributor.authorGul, Jasra
dc.contributor.authorAhmed, Usman
dc.contributor.authorShah, Muhammad Raza
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorYing, Tan Yee
dc.contributor.authorTong, Yeo Jia
dc.contributor.authorAnwar, Ayaz
dc.date.accessioned2025-04-16T19:40:09Z
dc.date.available2025-04-16T19:40:09Z
dc.date.issued2024
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü
dc.description.abstractAcanthamoeba castellanii is the causative pathogen of a severe eye infection, known as Acanthamoeba keratitis and a life-threatening brain infection, named granulomatous amoebic encephalitis. Current treatments are problematic and costly and exhibit limited efficacy against Acanthamoeba parasite, especially the cyst stage. In parallel to drug discovery and drug repurposing efforts, drug modification is also an important approach to tackle infections, especially against neglected parasites such as free-living amoebae: Acanthamoeba. In this study, we determined whether modifying pentamidine and doxycycline through chitosan-functionalized graphene oxide loading enhances their anti-amoebic effects. Various concentrations of doxycycline, pentamidine, graphene oxide, chitosan-functionalized graphene oxide, and chitosan-functionalized graphene oxide loaded with doxycycline and pentamidine were investigated for amoebicidal effects against pathogenic A. castellanii belonging to the T4 genotype. Lactate dehydrogenase assays were performed to determine toxic effects of these various drugs and nanoconjugates against human cells. The findings revealed that chitosan-functionalized graphene oxide loaded with doxycycline demonstrated potent amoebicidal effects. Nanomaterials significantly (p < 0.05) inhibited excystation and encystation of A. castellanii without exhibiting toxic effects against human cells in a concentration-dependent manner, as compared with other formulations. These results indicate that drug modifications coupled with nanotechnology may be a viable avenue in the rationale development of effective therapies against Acanthamoeba infections. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
dc.description.sponsorshipThis research work was supported by Sunway University, Malaysia, and University of Karachi, Pakistan. This research work was supported by Sunway University, Malaysia GRTIN-RRO-46\u20132022. Ruqaiyyah Siddiqui and Naveed Ahmed Khan are supported by the Air Force Office of Scientific Research (AFOSR), USA.
dc.identifier.citationJabri, T., Daalah, M., Alawfi, B. S., Gul, J., Ahmed, U., Shah, M. R., ... & Anwar, A. (2024). Drug modifications: graphene oxide-chitosan loading enhanced anti-amoebic effects of pentamidine and doxycycline. Parasitology Research, 123(11), 387.
dc.identifier.doi10.1007/s00436-024-08389-6
dc.identifier.issn09320113
dc.identifier.issue11
dc.identifier.scopusqualityQ1
dc.identifier.urihttp://dx.doi.org/10.1007/s00436-024-08389-6
dc.identifier.urihttps://hdl.handle.net/20.500.12713/6056
dc.identifier.volume123
dc.identifier.wosWOS:001360956400001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakScopus
dc.indekslendigikaynakWeb of Science
dc.institutionauthorKhan, Naveed Ahmed
dc.institutionauthorSiddiqui, Ruqaiyyah
dc.institutionauthoridNaveed Ahmed Khan / 0000-0001-7667-8553
dc.institutionauthoridRuqaiyyah Siddiqui / 0000-0001-9646-6208
dc.language.isoen
dc.publisherSpringer Science and Business Media Deutschland GmbH
dc.relation.ispartofParasitology Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcanthamoeba
dc.subjectDoxycycline
dc.subjectDrug Modification
dc.subjectGraphene Oxide
dc.subjectPentamidine
dc.titleDrug modifications: graphene oxide-chitosan loading enhanced anti-amoebic effects of pentamidine and doxycycline
dc.typeArticle

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