Drug modifications: graphene oxide-chitosan loading enhanced anti-amoebic effects of pentamidine and doxycycline

Basit Öğe Kaydı
dc.authoridTooba Jabri
dc.authoridMeshal Daalah
dc.authoridBader S. Alawfi
dc.authoridJasra Gul
dc.authoridUsman Ahmed
dc.authoridMuhammad Raza Shah
dc.authoridNaveed Ahmed Khan / 0000-0001-7667-8553
dc.authoridRuqaiyyah Siddiqui / 0000-0001-9646-6208
dc.authoridTan Yee Ying
dc.authoridYeo Jia Tong
dc.authoridAyaz Anwar
dc.authorscopusidNaveed Ahmed Khan / 58685542900
dc.authorwosidNaveed Ahmed Khan / AAM-2892-2021
dc.contributor.authorJabri, Tooba
dc.contributor.authorDaalah, Meshal
dc.contributor.authorAlawfi, Bader S.
dc.contributor.authorGul, Jasra
dc.contributor.authorAhmed, Usman
dc.contributor.authorShah, Muhammad Raza
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorYing, Tan Yee
dc.contributor.authorTong, Yeo Jia
dc.contributor.authorAnwar, Ayaz
dc.date.accessioned2025-04-18T09:26:01Z
dc.date.available2025-04-18T09:26:01Z
dc.date.issued28.11.2024
dc.departmentİstinye Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü
dc.description.abstractAcanthamoeba castellanii is the causative pathogen of a severe eye infection, known as Acanthamoeba keratitis and a life-threatening brain infection, named granulomatous amoebic encephalitis. Current treatments are problematic and costly and exhibit limited efficacy against Acanthamoeba parasite, especially the cyst stage. In parallel to drug discovery and drug repurposing efforts, drug modification is also an important approach to tackle infections, especially against neglected parasites such as free-living amoebae: Acanthamoeba. In this study, we determined whether modifying pentamidine and doxycycline through chitosan-functionalized graphene oxide loading enhances their anti-amoebic effects. Various concentrations of doxycycline, pentamidine, graphene oxide, chitosan-functionalized graphene oxide, and chitosan-functionalized graphene oxide loaded with doxycycline and pentamidine were investigated for amoebicidal effects against pathogenic A. castellanii belonging to the T4 genotype. Lactate dehydrogenase assays were performed to determine toxic effects of these various drugs and nanoconjugates against human cells. The findings revealed that chitosan-functionalized graphene oxide loaded with doxycycline demonstrated potent amoebicidal effects. Nanomaterials significantly (p < 0.05) inhibited excystation and encystation of A. castellanii without exhibiting toxic effects against human cells in a concentration-dependent manner, as compared with other formulations. These results indicate that drug modifications coupled with nanotechnology may be a viable avenue in the rationale development of effective therapies against Acanthamoeba infections.
dc.identifier.citationJabri, T., Daalah, M., Alawfi, B. S., Gul, J., Ahmed, U., Shah, M. R., ... & Anwar, A. (2024). Drug modifications: graphene oxide-chitosan loading enhanced anti-amoebic effects of pentamidine and doxycycline. Parasitology Research, 123(11), 387.
dc.identifier.doi10.1007/s00436-024-08389-6
dc.identifier.endpage187
dc.identifier.issn0932-0113
dc.identifier.issnhttps://doi.org/10.1007/s00436-024-08389-6
dc.identifier.pmid39565414
dc.identifier.scopus2-s2.0-85209758458
dc.identifier.scopusqualityQ1
dc.identifier.startpage123
dc.identifier.urihttps://www.webofscience.com/wos/woscc/full-record/WOS:001360956400001
dc.identifier.urihttps://hdl.handle.net/20.500.12713/6789
dc.identifier.wosWOS:001360956400001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKhan, Naveed Ahmed
dc.institutionauthorSiddiqui, Ruqaiyyah
dc.institutionauthoridNaveed Ahmed Khan / 0000-0001-7667-8553
dc.institutionauthoridRuqaiyyah Siddiqui / 0000-0001-9646-6208
dc.language.isoen
dc.publisherSPRINGER
dc.relation.ispartofPARASITOLOGY RESEARCH
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectACANTHAMOEBA SPP.ORAL DELIVERYWATERNANOPARTICLESMECHANISMDISEASEBRAIN
dc.titleDrug modifications: graphene oxide-chitosan loading enhanced anti-amoebic effects of pentamidine and doxycycline
dc.typeArticle

Dosyalar

Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.17 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Eczacılık Meslek Bilimleri Bölümü Makale Koleksiyonu