In vitro cytopathogenic activities of acanthamoeba t3 and t4 genotypes on hela cell monolayer

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dc.authoridRuqaiyyah Siddiqui / 0000-0001-9646-6208en_US
dc.authoridNaveed Khan / 0000-0001-7667-8553en_US
dc.authorscopusidRuqaiyyah Siddiqui / 18635417000en_US
dc.authorscopusidNaveed Ahmedi Khan / 7401797675en_US
dc.authorwosidRuqaiyyah Siddiqui / FVT-1496-2022en_US
dc.authorwosidNaveed Ahmedi Khan / FXL-5617-2022en_US
dc.contributor.authorHussain, Rosnani Hanim Mohd
dc.contributor.authorGhani, Mohamed Kamel Abdul
dc.contributor.authorKhan, Naveed Ahmed
dc.contributor.authorSiddiqui, Ruqaiyyah
dc.contributor.authorAazmi, Shafiq
dc.contributor.authorHalim, Hasseri
dc.contributor.authorAnuar, Tengku Shahrul
dc.date.accessioned2023-01-26T12:43:20Z
dc.date.available2023-01-26T12:43:20Z
dc.date.issued2022en_US
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractAmoebic keratitis and encephalitis are mainly caused by free-living amoebae of the genus Acanthamoeba, which consists of both pathogenic and nonpathogenic species. The global distribution, amphizoic properties and the severity of the disease caused by Acanthamoeba species have inspired the scientific community to put more effort into the isolation of Acanthamoeba, besides exploring the direct and indirect parameters that could signify a pathogenic potential. Therefore, this study was performed to characterize the pathogenic potential of Acanthamoeba isolated from contact lens paraphernalia and water sources in Malaysia. Various methodologies were utilized to analyze the thermotolerance and osmotolerance, the secretion level of proteases and the cytopathic effect of trophozoites on the cell monolayer. In addition, the in vitro cytopathogenicity of these isolates was assessed using the LDH-release assay. A total of 14 Acanthamoeba isolates were classified as thermo- and osmotolerant and had presence of serine proteases with a molecular weight of 45-230 kDa. Four T4 genotypes isolated from contact lens paraphernalia recorded the presence of serine-type proteases of 107 kDa and 133 kDa. In contrast, all T3 genotypes isolated from environmental samples showed the presence of a 56 kDa proteolytic enzyme. Remarkably, eight T4 and a single T3 genotype isolates demonstrated a high adhesion percentage of greater than 90%. Moreover, the use of the HeLa cell monolayer showed that four T4 isolates and one T3 isolate achieved a cytopathic effect in the range of 44.9-59.4%, indicating an intermediate-to-high cytotoxicity level. Apart from that, the LDH-release assay revealed that three T4 isolates (CL5, CL54 and CL149) and one T3 isolate (SKA5-SK35) measured an exceptional toxicity level of higher than 40% compared to other isolates. In short, the presence of Acanthamoeba T3 and T4 genotypes with significant pathogenic potential in this study reiterates the essential need to reassess the functionality of other genotypes that were previously classified as nonpathogenic isolates in past research.en_US
dc.identifier.citationMohd Hussain, R. H., Abdul Ghani, M. K., Khan, N. A., Siddiqui, R., Aazmi, S., Halim, H., & Anuar, T. S. (2022). In Vitro Cytopathogenic Activities of Acanthamoeba T3 and T4 Genotypes on HeLa Cell Monolayer. Pathogens, 11(12), 1474.en_US
dc.identifier.doi10.3390/pathogens11121474en_US
dc.identifier.issn2076-0817en_US
dc.identifier.issue12en_US
dc.identifier.urihttp://dx.doi.org/110.3390/pathogens11121474
dc.identifier.urihttps://hdl.handle.net/20.500.12713/3844
dc.identifier.volume11en_US
dc.identifier.wosWOS:000903239200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorKhan, Naveed Ahmed
dc.institutionauthorSiddiqui, Ruqaiyyah
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofPATHOGENSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAcanthamoebaen_US
dc.subjectCytopathic Effecten_US
dc.subjectHela Cellsen_US
dc.subjectPathogenic Potentialen_US
dc.subjectProteaseen_US
dc.titleIn vitro cytopathogenic activities of acanthamoeba t3 and t4 genotypes on hela cell monolayeren_US
dc.typeArticleen_US

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