MicroRNA-200c overexpression in cancer-associated fibroblasts decreases interleukin-2 secretion

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dc.contributor.authorShariati, L.
dc.contributor.authorVaseghi, G.
dc.contributor.authorVaziri, N.
dc.contributor.authorShenavar, N.
dc.contributor.authorZarrabi, A.
dc.contributor.authorHaghjooy, Javanmard, S.
dc.date.accessioned2024-05-19T14:33:31Z
dc.date.available2024-05-19T14:33:31Z
dc.date.issued2022
dc.departmentİstinye Üniversitesien_US
dc.description.abstractmiR-200c-3p is demonstrated to play the role of tumour suppressor in different tumours. However, the miR-200c-3p biological function in normal fibroblast (NF) and cancer-associated fibroblast (CAF) remains unclear. This investigation aims to study the regulatory role of miR-200c-3p in the secretion of Interleukin-2 (IL-2) in CAF and NF. CAFs and NFs were isolated from tumour and normal tissue specimens respectively. Immunocytochemistry was used to confirm the presence of a fibroblast specific marker, alpha-actin smooth muscle, in NFs and CAFs. NF and CAF were transfected with scramble and miR-200c-3p utilizing the lipofectamine 2000 reagent. The protein levels of IL-2 were measured in CAFs, NFs, and transfected groups with miR-200c-3p and scrambled using an IL-2 enzyme-linked immunoassay kit. miR-200c decreased secretion of IL-2 in transfected CAF and NF compared to controls. Results elucidated that transfection of MiR-200c-3p can decrease the IL-2 secretion and consequently reduce IL-induced tumourigenic manner in the CAF. © 2022 The Authors. Clinical and Translational Discovery published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics.en_US
dc.description.sponsorshipIsfahan University of Medical Sciences, IUMS: 297184en_US
dc.description.sponsorshipThis work was supported by Isfahan University of Medical Sciences [grant number 297184].en_US
dc.identifier.doi10.1002/ctd2.90
dc.identifier.issn2768-0622
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85165482850en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1002/ctd2.90
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4260
dc.identifier.volume2en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBlackwell Publishingen_US
dc.relation.ispartofClinical and Translational Discoveryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectBreast Canceren_US
dc.subjectCancer-Associated Fibroblasten_US
dc.subjectMir-200c-3pen_US
dc.subjectNormal Fibroblasten_US
dc.titleMicroRNA-200c overexpression in cancer-associated fibroblasts decreases interleukin-2 secretionen_US
dc.typeArticleen_US

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