Comparıson of cumulus cells and follıcular fluıd obtaıned from ınfertile ındıvıduals dıagnosed wıth polycystıc ovary syndrome (PCOS) and endomethrıosıs wıth samples obtaıned from healthy ındıvıduals

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dc.contributor.authorGürgan, Timur
dc.contributor.authorRuso, Halil
dc.contributor.authorTural, Rabia
dc.contributor.authorDinçel, Aylin Sepici
dc.contributor.authorDayanır, Duygu
dc.contributor.authorKalem, Ziya
dc.contributor.authorSarıbaş, Sanem
dc.date.accessioned2024-05-19T14:23:35Z
dc.date.available2024-05-19T14:23:35Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractAim: Investigating the relationship between Growth differentiation factor-9 (GDF-9), Bone morphogenetic protein-15 (BMP-15) markers, apoptosis levels in cumulus cells and total oxidant (TOS)/ anti-oxidant (TAS) stress levels, inflammation parameters (interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-alpha)) in follicular fluid belonging to patients with polycystic ovary syndrome (PCOS), endometriosis (END) and male factor (MF) (control) groups. Material and Methods: GDF-9 and BMP-15 markers are determined by immunohistochemical methods, apoptosis levels are studied with TUNEL. TOS and TAS statuses are investigated with spectrophotometry, IL-6 and TNF – alpha levels are examined by Enzyme-Linked Immuno Sorbent Assay (ELISA). Results: According to the data obtained in the study; GDF-9 and BMP-15 levels are found to be lower in PCOS and END groups and apoptosis levels of cumulus cells were significantly higher at these groups . TOS levels were significantly higher in PCOS and END groups whereas follicular fluid TAS levels were not statistically significant for these groups. IL-6 and TNF – alpha levels of follicular fluid was significantly higher in PCOS. These parameters were also higher for END group, however the difference was not found to be significant. Conclusion: Our results imply that correlation between GDF-9, BMP-15 markers, apotosis levels, oxidative status, inflammation levels may be interpreted with improper environment for oocyte maturation for patients diagnosed with PCOS or END. The need for further studies on subject proceeds. However, if similar datas are obtained in further studies, it is thought that evaluation of cumulus cell properties together with especially follicular fluid oxidative stress levels will contribute to the selection of the best oocyte.en_US
dc.identifier.doi10.18663/tjcl.1339043
dc.identifier.endpage586en_US
dc.identifier.issn2149-8296
dc.identifier.issue3en_US
dc.identifier.startpage576en_US
dc.identifier.trdizinid1200423en_US
dc.identifier.urihttps://doi.org/10.18663/tjcl.1339043
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1200423
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4044
dc.identifier.volume14en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Clinics and Laboratoryen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.titleComparıson of cumulus cells and follıcular fluıd obtaıned from ınfertile ındıvıduals dıagnosed wıth polycystıc ovary syndrome (PCOS) and endomethrıosıs wıth samples obtaıned from healthy ındıvıdualsen_US
dc.typeArticleen_US

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