In vivo Gonadoprotective Effects of Myricetin on Cisplatin- Induced Testicular Damage via Suppression of TLR4/NF-?B Inflammation Pathway and Heat-Shock Response

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dc.contributor.authorAkin, Ali Tugrul
dc.date.accessioned2024-05-19T14:40:08Z
dc.date.available2024-05-19T14:40:08Z
dc.date.issued2023
dc.departmentİstinye Üniversitesien_US
dc.description.abstractThe aim of this study is to reveal the gonadoprotective effects of myricetin (MYC), which has many biological properties, on cisplatin (CP)-induced testicular damage in rats. For this purpose, 40 male Wistar albino rats were divided into 4 groups as Control (group given no treatment), MYC (group given 5 mg/kg/i.p myricetin for 7 days), CP (group given 7 mg/kg/i.p cisplatin at 7th day) and MYC + CP (group given 5 mg/kg/i.p myricetin for 7 days before 7 mg/kg/i.p cisplatin injection). After administrations, testicular tissues of animals were extracted and processed according to tissue processing protocol. Hematoxylin & Eosin staining were performed to evaluate the histopathological changes and Johnsen'sTesticular Biopsy Score (JTBS) was applied and mean seminiferous tubule diameters (MSTD) were measured to compare experimental groups in terms of histopathological changes. Moreover, TLR4, NF-kappa B, HSP70 and HSP90 expression levels were detected by immunohistochemical staining and the density of immunoreactivity were measured to determine the difference in the expression levels of these factors among groups. Additionally, testicular apoptosis was detected via TUNEL assay. JTBS and MSTD data were significantly lower in CP group compared to other groups and MYC administrations significantly protects testicular tissue against CP-induced damage. Moreover, TLR4, NF-kappa B, HSP70 and HSP90 expressions and apoptotic cells significantly increased in the CP group (p<0.05). However, MYC administrations exerted a strong gonadoprotective effect on testicular tissue in terms of these parameters in MYC+CP group (p<0.05). According to our results, we suggested that MYC can be considered as a protective agent against cisplatin-induced testicular damage.en_US
dc.identifier.endpage1880en_US
dc.identifier.issn0717-9502
dc.identifier.issn0717-9367
dc.identifier.issue6en_US
dc.identifier.startpage1870en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4911
dc.identifier.volume41en_US
dc.identifier.wosWOS:001132665600012en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSoc Chilena Anatomiaen_US
dc.relation.ispartofInternational Journal of Morphologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240519_kaen_US
dc.subjectCisplatinen_US
dc.subjectHeat-Shock Responseen_US
dc.subjectInflammationen_US
dc.subjectMyricetinen_US
dc.subjectTesticular Damageen_US
dc.titleIn vivo Gonadoprotective Effects of Myricetin on Cisplatin- Induced Testicular Damage via Suppression of TLR4/NF-?B Inflammation Pathway and Heat-Shock Responseen_US
dc.typeArticleen_US

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