Protective effect of Apelin-13 on D-glutamic acid-induced excitotoxicity in SH-SY5Y cell line: An in-vitro study

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dc.authorscopusidKadriye Yağmur Oruç / 58187315000
dc.authorwosidKadriye Yağmur Oruç / AAR-9552-2020
dc.contributor.authorOruç, Kadriye Yağmur
dc.contributor.authorAğtürk, Gökhan
dc.contributor.authorOruç, Aykut
dc.contributor.authorYanar, Karolin
dc.contributor.authorSeymen, Hakkı Oktay
dc.date.accessioned2025-04-18T10:26:21Z
dc.date.available2025-04-18T10:26:21Z
dc.date.issued2025
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractExcitotoxicity, resulting from excessive accumulation of glutamate in the extracellular space, leads to neuronal cell death. This study investigates the protective effects of Apelin-13 on D-Glutamic acid-induced excitotoxicity in SH-SY5Y human neuroblastoma cells, an in-vitro model for neurodegenerative diseases. Unlike the commonly studied L-glutamic acid, this research focuses on D-Glutamic acid to understand its specific impacts. SH-SY5Y cells were treated with varying concentrations of D-Glutamic acid and Apelin-13, followed by analyses at 12 and 24 h to evaluate cell viability, oxidative stress markers, and inflammatory cytokine levels. Cell viability assays revealed significant cytotoxic effects of D-Glutamic acid at doses of 10 mM and 20 mM, reducing viability by over 50 %. However, Apelin-13 treatment mitigated these effects, especially at 2 μg/ml, enhancing cell viability and reducing inflammatory cytokine levels (IL-1β and TNF-α). Apelin-13 also increased anti-inflammatory cytokine levels (IL-10 and TGF-β1) and brain-derived neurotrophic factor (BDNF), indicating its neuroprotective role. Oxidative stress markers, including ROS, AGE, AOPP, DT, T-SH, were significantly elevated by D-Glutamic acid but effectively reduced by Apelin-13. The neuroprotective mechanisms of Apelin-13 involve modulation of cAMP/PKA and MAPK signaling pathways, enhancing BDNF synthesis and suppressing oxidative stress and inflammatory responses. This study is the first to demonstrate the effects of D-Glutamic acid on SH-SY5Y cells. It highlights Apelin-13's potential as a therapeutic agent against excitotoxicity-induced neuronal damage, emphasizing its ability to modulate key molecular pathways involved in inflammation and oxidative stress. Further in-vivo studies are warranted to explore the long-term neuroprotective effects of Apelin-13 in treating neurodegenerative diseases. © 2024
dc.description.sponsorshipThis study was supported by Istanbul University-Cerrahpa\u015Fa Scientific Research Projects Unit (Project grant number: TSA-2023-37359). Funding text 2 This study was supported by Istanbul University -Cerrahpa\u015Fa Scientific Research Projects Unit (Project grant number: TSA-2023-37,359 ).
dc.identifier.citationOruç, K. Y., Ağtürk, G., Oruç, A., Yanar, K., & Seymen, H. O. (2025). Protective effect of Apelin-13 on D-glutamic acid-induced excitotoxicity in SH-SY5Y cell line: An in-vitro study. Neuropeptides, 109, 102483.
dc.identifier.doi10.1016/j.npep.2024.102483
dc.identifier.issn01434179
dc.identifier.scopus2-s2.0-85208764156
dc.identifier.scopusqualityQ2
dc.identifier.urihttp://dx.doi.org/10.1016/j.npep.2024.102483
dc.identifier.urihttps://hdl.handle.net/20.500.12713/7070
dc.identifier.volume109
dc.indekslendigikaynakScopus
dc.institutionauthorOruç, Kadriye Yağmur
dc.institutionauthoridKadriye Yağmur Oruç / 0000-0002-3747-5136
dc.language.isoen
dc.publisherChurchill Livingstone
dc.relation.ispartofNeuropeptides
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectApelin-13
dc.subjectD-glutamic Acid
dc.subjectExcitotoxicity
dc.subjectNeuroinflammation
dc.subjectNeurotoxicity
dc.subjectSH-SY5Y Cell Line
dc.titleProtective effect of Apelin-13 on D-glutamic acid-induced excitotoxicity in SH-SY5Y cell line: An in-vitro study
dc.typeArticle

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