The apoptotic effect of garlic (Allium sativum) derived SEVs on different types of cancer cell lines in vitro

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dc.authorscopusidPolen Koçak / 59210802100
dc.authorwosidPolen Koçak / KXD-5469-2024
dc.contributor.authorÜnsal, Naz
dc.contributor.authorKoçak, Polen
dc.contributor.authorYılmaz, Hazal
dc.contributor.authorŞahin, Fikrettin
dc.contributor.authorCanpolat, Merve Yıldırım
dc.date.accessioned2025-04-18T10:09:37Z
dc.date.available2025-04-18T10:09:37Z
dc.date.issued2024
dc.departmentİstinye Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Biyomedikal Mühendisliği Bölümü
dc.description.abstractBackground/aim: Small extracellular vesicles (SEVs) are known to have an impact on the physiological conditions of target cells, are a critical component of cell -to -cell communication, and have been implicated in a variety of diseases. Although it has been proposed that edible plant -derived nanoparticles have an effect on communication with mammalian cells, the influence of these nanoparticles on cancer cell development has yet to be explored. Materials and methods: In order to characterize small extracellular vesicles obtained from garlic, specific SEV surface markers, antibodies, and size detections were identified using scanning electron microscopy and nanoparticle tracking analysis. Human hepatoma (Hep3B), human neuroblastoma (SH-SY5Y), human pancreatic adenocarcinoma (Panc-1a), human glioblastoma (U87), prostate cancer (PC -3), and human umbilical vein endothelial (HUVEC) cell lines were treated with garlic SEVs to examine their anticancer properties. Results: Annexin V FITC/PI staining for apoptosis, mRNA, and protein expression levels via RT-PCR and ELISA indicated that garlic SEVs triggered apoptosis by activating the intrinsic pathway. Our findings support the idea that SEVs produced from garlic may trigger apoptotic cell death in cancer cells while having no effect on healthy cells. Conclusion: It was discovered that plant SEVs had anti -cancer effects by activating caspase-mediated apoptosis.
dc.description.sponsorshipYeditepe University
dc.identifier.citationÜnsal, N., KOÇAK DENİZCİ, P. O. L. E. N., Yilmaz, H., Şahin, F., & YILDIRIM CANPOLAT, M. E. R. V. E. (2024). The apoptotic effect of garlic (Allium sativum) derived SEVs on different types of cancer cell lines in vitro. Turkish Journal of Biology, 48(3), 182-191.
dc.identifier.doi10.55730/1300-0152.2694
dc.identifier.endpage191
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue3
dc.identifier.pmid39050709
dc.identifier.scopus2-s2.0-85197886828
dc.identifier.scopusqualityQ1
dc.identifier.startpage182
dc.identifier.urihttp://dx.doi.org/10.55730/1300-0152.2694
dc.identifier.urihttps://hdl.handle.net/20.500.12713/6968
dc.identifier.volume48
dc.identifier.wosWOS:001260244800003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKoçak, Polen
dc.institutionauthoridPolen Koçak / 0000-0003-4493-1061
dc.language.isoen
dc.publisherTubitak scientific & technological research council Turkey
dc.relation.ispartofTurkish journal of biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPlant-Derived Extracellular Vesicles
dc.subjectApoptosis
dc.subjectCancer
dc.titleThe apoptotic effect of garlic (Allium sativum) derived SEVs on different types of cancer cell lines in vitro
dc.typeArticle

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