Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression

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dc.authoridSevgın Degırmencıoglu / 0000-0001-7243-3671
dc.authoridPınar Çetinalp / 0000-0001-8105-0322
dc.authoridSevda Tanrıkulu Küçük / 0000-0002-1173-7759
dc.authoridMuhammed Seyithanoğlu / 0000-0002-8027-7549
dc.authoridHikmet Kocak / 0000-0003-1720-9102
dc.authoridYıldız Oner-Iyidogan / 0000-0001-6956-8794
dc.authorscopusidHikmet Kocak / 58913661500
dc.authorwosidHikmet Kocak / AAQ-7520-2020
dc.contributor.authorDegırmencıoglu, Sevgın
dc.contributor.authorÇetinalp, Pınar
dc.contributor.authorKüçük, Sevda Tanrıkulu
dc.contributor.authorSeyithanoğlu, Muhammed
dc.contributor.authorKocak, Hikmet
dc.contributor.authorIyidogan, Yıldız Oner
dc.date.accessioned2025-04-18T10:19:34Z
dc.date.available2025-04-18T10:19:34Z
dc.date.issued03.11.2024
dc.departmentİstinye Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü
dc.description.abstractObjective: Adipose tissue stores lipids necessary for the maintenance of nutritional homeostasis. It is also an endocrine organ that reacts to changes in inflammation and energy status. Capsaicin, the principal bioactive compound in red pepper, has garnered significant attention for its reported anti-obesity, anti-diabetic, anti-oxidant, and anti-inflammatory properties. In this study, we aimed to elucidate the influence and most efficacious dose of capsaicin on the expression of lipid metabolism-related inflammatory proteins and the inhibition of adipocyte cell differentiation. Materials and Methods: Cell viability analysis was performed using CCK-8, cell differentiation was assessed using Oil Red O, and gene expression levels of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding protein alpha (C/EBPα), adiponectin, leptin, cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), nuclear factor kappa B1 (NF-κB1), tumor necrosis factor-alpha (TNF-α), sirtuin-1 (SIRT-1), transient receptor potential vanilloid receptor 1 (TRPV1), and uncoupling protein 2 (UCP2) were evaluated using quantitative real time polymerase chain reaction (qRT-PCR). Statistical analyses were conducted using GraphPad Prism 5. One-way ANOVA was performed to compare quantitative data between the groups. Results: Capsaicin suppressed preadipocyte-to-adipocyte differentiation and mitigated the release of pro-inflammatory cytokines, particularly at low concentrations. Capsaicin effectively suppressed adiponectin levels at all concentrations but decreased leptin levels at lower concentrations (0.5 μM and 1 μM). Capsaicin stimulated the expressions of SIRT1 and TRPV-1 in adipocytes. According to our findings, the most effective capsaicin dose for the regulation of SIRT1 and TRPV-1 expressions appears to be 20 μM. Conclusion: Capsaicin’s effect on proteins regulating adipogenesis is not dose-related, but its inhibitory effect on adiposity-dependent inflammation was more pronounced at low concentrations.
dc.identifier.citationDEGIRMENCIOGLU, S., ÇETINALP, P., KÜÇÜK, S. T., SEYITHANOĞLU, M., KOCAK, H., ONER-IYIDOGAN, Y. (2024). Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression. EXPERIMED , 14(2), 116 - 125. doi.org/10.26650/experimed.1489790
dc.identifier.doi10.26650/experimed.1489790
dc.identifier.endpage125
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85202881543
dc.identifier.startpage116
dc.identifier.trdizinid1277165
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1277165/capsaicin-modulates-adipocyte-cell-differentiation-and-inflammatory-gene-expression
dc.identifier.urihttps://hdl.handle.net/20.500.12713/7027
dc.identifier.volume14
dc.identifier.wosWOS:001322096300008
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorKocak, Hikmet
dc.institutionauthoridHikmet Kocak / 0000-0003-1720-9102
dc.language.isoen
dc.publisherİstanbul Üniversitesi
dc.relation.ispartofEXPERIMED
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAdipogenesis
dc.subjectcapsaicin
dc.subjectcytokines
dc.subjectdifferentiation and inflammation
dc.titleCapsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression
dc.typeArticle

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