Serum multi-omics analysis in hindlimb unloading mice model: Insights into systemic molecular changes and potential diagnostic and therapeutic biomarkers

Basit Öğe Kaydı
dc.contributor.authorIbrahim, Z.
dc.contributor.authorKhan, N.A.
dc.contributor.authorQaisar, R.
dc.contributor.authorSaleh, M.A.
dc.contributor.authorSiddiqui, R.
dc.contributor.authorAl-Hroub, H.M.
dc.contributor.authorGiddey A.D.
dc.date.accessioned2024-05-19T14:33:29Z
dc.date.available2024-05-19T14:33:29Z
dc.date.issued2024
dc.departmentİstinye Üniversitesien_US
dc.description.abstractMicrogravity, in space travel and prolonged bed rest conditions, induces cardiovascular deconditioning along with skeletal muscle mass loss and weakness. The findings of microgravity research may also aid in the understanding and treatment of human health conditions on Earth such as muscle atrophy, and cardiovascular diseases. Due to the paucity of biomarkers and the unknown underlying mechanisms of cardiovascular and skeletal muscle deconditioning in these environments, there are insufficient diagnostic and preventative measures. In this study, we employed hindlimb unloading (HU) mouse model, which mimics astronauts in space and bedridden patients, to first evaluate cardiovascular and skeletal muscle function, followed by proteomics and metabolomics LC-MS/MS-based analysis using serum samples. Three weeks of unloading caused changes in the function of the cardiovascular system in c57/Bl6 mice, as seen by a decrease in mean arterial pressure and heart weight. Unloading for three weeks also changed skeletal muscle function, causing a loss in grip strength in HU mice and atrophy of skeletal muscle indicated by a reduction in muscle mass. These modifications were partially reversed by a two-week recovery period of reloading condition, emphasizing the significance of the recovery process. Proteomics analysis revealed 12 dysregulated proteins among the groups, such as phospholipid transfer protein, Carbonic anhydrase 3, Parvalbumin alpha, Major urinary protein 20 (Mup20), Thrombospondin-1, and Apolipoprotein C-IV. On the other hand, metabolomics analysis showed altered metabolites among the groups such as inosine, hypoxanthine, xanthosine, sphinganine, L-valine, 3,4-Dihydroxyphenylglycol, and L-Glutamic acid. The joint data analysis revealed that HU conditions mainly impacted pathways such as ABC transporters, complement and coagulation cascades, nitrogen metabolism, and purine metabolism. Overall, our results indicate that microgravity environment induces significant alterations in the function, proteins, and metabolites of these mice. These observations suggest the potential utilization of these proteins and metabolites as novel biomarkers for assessing and mitigating cardiovascular and skeletal muscle deconditioning associated with such conditions. © 2023 The Authorsen_US
dc.description.sponsorshipAir Force Office of Scientific Research, AFOSR: FA9550-23-1-0711; University of Sharjah, UOSen_US
dc.description.sponsorshipThis project is partialy supported by Cardiovascular Diseases Research Group and Space Medicine Research Group Operational Grants, University of Sharjah (UOS) . Zeinab Ibrahim is supported by College of Graduate Studies and Scientific Research, UOS . Ruqaiyyah Siddiqui and Naveed Ahmed Khan are supported by the Air Force Office of Scientific Research (AFOSR), USA Grant number: FA9550-23-1-0711 .en_US
dc.identifier.doi10.1016/j.heliyon.2023.e23592
dc.identifier.issn2405-8440
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85179967807en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.heliyon.2023.e23592
dc.identifier.urihttps://hdl.handle.net/20.500.12713/4249
dc.identifier.volume10en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofHeliyonen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240519_kaen_US
dc.subjectBiomarkersen_US
dc.subjectCardiovascularen_US
dc.subjectDeconditioningen_US
dc.subjectHindlimb Unloadingen_US
dc.subjectMetabolomicen_US
dc.subjectProteomicen_US
dc.subjectSkeletal Muscleen_US
dc.titleSerum multi-omics analysis in hindlimb unloading mice model: Insights into systemic molecular changes and potential diagnostic and therapeutic biomarkersen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu